Islam A
Leuk Res. 1985;9(11):1415-32. doi: 10.1016/0145-2126(85)90130-4.
It is generally accepted that morphologically recognizable bone marrow (BM) cells are derived from progenitor cells committed to a specific line of haematopoietic differentiation [51]. The origin and morphological identity of such progenitor cells is not yet known and the question, whether there is a single pluripotent haematopoietic stem cell (HSC) or a variety of stem cells each with a capacity of self-replication and maintenance is still unresolved [37]. Although progress has been made in segregating [43, 47, 49, 64] various progenitor cells from one another and functional assays [11, 17, 42] have significantly promoted the study of haemopoietic precursor cells, morphological investigations were less rewarding. Even after enrichment, the morphology of haemopoietic stem- and colony-forming cells only remained hypothetical, 'candidate stem cells' [3], because their precursor cell qualities could not be deduced from their morphology, but only retrospectively, and statistically from their function, i.e. in vitro colony-formation or in-vivo haemopoietic reconstitution from an enriched cell suspension [58]. We postulate from our studies on semi-thin sections of undecalcified BM from healthy human fetuses, normal adults and patients with acute myeloid leukaemia (AML) and chronic granulocytic leukaemia (CGL) that the endosteal cells are the precursors of all haematopoietic cells in the bone marrow and are also capable of transformation into either stromal (fibroblast-like cells) or bone-related cells (osteoblasts and osteoclasts).
一般认为，形态上可识别的骨髓（BM）细胞源自致力于特定造血分化谱系的祖细胞[51]。此类祖细胞的起源和形态特征尚不清楚，关于是否存在单一的多能造血干细胞（HSC）或多种各自具有自我复制和维持能力的干细胞这一问题仍未解决[37]。尽管在将各种祖细胞彼此分离方面已取得进展[43, 47, 49, 64]，并且功能测定[11, 17, 42]显著促进了造血前体细胞的研究，但形态学研究的收获较少。即使经过富集，造血干细胞和集落形成细胞的形态仍只是假设的“候选干细胞”[3]，因为它们的前体细胞特性无法从其形态推断出来，而只能通过回顾性研究，并从其功能进行统计学推断，即通过体外集落形成或从富集的细胞悬液进行体内造血重建来推断[58]。我们通过对健康人类胎儿、正常成年人以及急性髓性白血病（AML）和慢性粒细胞白血病（CGL）患者的未脱钙骨髓半薄切片进行研究后推测，骨内膜细胞是骨髓中所有造血细胞的前体，并且也能够转化为基质细胞（成纤维样细胞）或骨相关细胞（成骨细胞和破骨细胞）。
