Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA.
Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA; City of Hope Comprehensive Cancer Center, Los Angeles, CA 91010, USA.
STAR Protoc. 2024 Sep 20;5(3):103096. doi: 10.1016/j.xpro.2024.103096. Epub 2024 Jul 31.
Type 2 innate lymphoid cells (ILC2s) are crucial in regulating immune responses and various physiological processes, including tissue repair, metabolic homeostasis, inflammation, and cancer surveillance. Here, we present a protocol that outlines the isolation, expansion, and adoptive transfer of human ILC2s from peripheral blood mononuclear cells for an in vivo lineage tracking experiment in a mouse model. Additionally, we detail the steps involved in the adoptive transfer of human ILC2s to recipient mice bearing human liquid or solid tumors. For complete details on the use and execution of this protocol, please refer to Li et al..
2 型固有淋巴细胞 (ILC2s) 在调节免疫反应和各种生理过程中至关重要，包括组织修复、代谢稳态、炎症和癌症监测。在这里，我们提供了一个从外周血单核细胞中分离、扩增和过继转移人 ILC2s 的方案，用于在小鼠模型中进行体内谱系追踪实验。此外，我们还详细介绍了将人 ILC2s 过继转移到携带人液体或实体瘤的受体小鼠中的步骤。有关使用和执行此方案的完整详细信息，请参阅 Li 等人的研究。
