用于无淋巴细胞小鼠重建的骨髓固有淋巴细胞分离方案。
A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution.
机构信息
Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2M9, Canada.
The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia.
出版信息
STAR Protoc. 2022 Sep 16;3(3):101534. doi: 10.1016/j.xpro.2022.101534. Epub 2022 Jul 12.
Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting. For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016).
先天淋巴细胞 (ILCs) 和适应性 T 细胞的转录组谱有显著重叠，因此研究它们仍然具有挑战性。在这里，我们描述了将 ILC 祖细胞过继转移到先天和适应性免疫细胞基因缺失的小鼠中，以允许在体内环境中对 ILCs 的发育和功能以及基因调控进行详细研究。有关此方案使用和执行的完整详细信息，请参见 Jacquelot 等人（2021 年）和 Seillet 等人（2016 年）的文章。
Zotero 插件