College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Harbin 150030, China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, China.
Ecotoxicol Environ Saf. 2024 Sep 15;283:116784. doi: 10.1016/j.ecoenv.2024.116784. Epub 2024 Jul 31.
2-ethylhexyl diphenyl phosphate (EHDPHP) is a widely used organophosphorus flame retardant and plasticizer, which is commonly found in the environment. EHDPHP not only potentially harms the environment but also causes different degrees of damage to the organism. In this study, the duodenum of chicks was selected as the potential toxic target organ to explore the mechanism of duodenal injury induced by EHDPHP exposure. Ninety one-day-old healthy male chicks were selected and randomly divided into C1(control group), C2(solvent control group), L(800 mg/kg), M(1600 mg/kg), H(3200 mg/kg) according to different doses of EHDPHP after one week of environmental adaptation. The chicks were given continuous gavage for 14 d, 28 d, and 42 d. It was found that constant exposure to EHDPHP caused an increase in duodenal MDA content, a decrease in P-gp, SOD, GSH-Px activities, and a decrease in duodenal mucosal immune factor (sIgA, GSH-Px). The expression of sIgM and mucosal link proteins (CLDN, OCLN, ZO-1, JAM) decreased, and the expression of the inflammatory protein (NF-κB, COX2) in duodenal tissues was up-regulated. The results showed that continuous exposure to EHDPHP could cause duodenal oxidative stress, inflammation, and mucosal barrier damage in chicks, which provided a basis for studying the mechanism of toxic damage caused by EHDPHP in poultry.
2-乙基己基二苯基磷酸酯（EHDPHP）是一种广泛使用的有机磷阻燃剂和增塑剂，普遍存在于环境中。EHDPHP 不仅对环境具有潜在危害，而且对生物体造成不同程度的损害。本研究以小鸡的十二指肠为潜在的毒性靶器官，探索 EHDPHP 暴露致十二指肠损伤的作用机制。选择 90 日龄健康雄性小鸡，经过 1 周环境适应后，根据 EHDPHP 不同剂量随机分为 C1（对照组）、C2（溶剂对照组）、L（800mg/kg）、M（1600mg/kg）、H（3200mg/kg）4 个组，连续灌胃 14、28、42d。结果发现，持续暴露于 EHDPHP 会引起十二指肠 MDA 含量增加，P-gp、SOD、GSH-Px 活性降低，十二指肠黏膜免疫因子（sIgA、GSH-Px）减少，黏膜连接蛋白（CLDN、OCLN、ZO-1、JAM）和黏膜免疫因子（sIgM）表达减少，十二指肠组织中炎症蛋白（NF-κB、COX2）表达上调。表明持续暴露于 EHDPHP 可引起雏鸡十二指肠氧化应激、炎症和黏膜屏障损伤，为研究 EHDPHP 对家禽的毒性损伤机制提供了依据。
