School of Chemical Engineering, University of New South Wales, Sydney, NSW, Australia.
Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, Australia.
Food Chem. 2024 Dec 1;460(Pt 2):140621. doi: 10.1016/j.foodchem.2024.140621. Epub 2024 Jul 24.
Cellular agriculture, an emerging technology, aims to produce animal-based products such as meat through scalable tissue culture methods. Traditional techniques rely on chemically undefined media using fetal bovine serum (FBS) or chemically defined media utilizing specific growth factors. To be a viable alternative to conventional meat production, cellular agriculture requires cost-effective materials with established supply chains for growth media. Here, we investigate hydrolysates from Kikuyu grass, Alfalfa grass, and cattle rearing pellets. We identified conditions that promote C2C12 myoblast cell growth in media containing 0.1% and 0% serum. These effects are more pronounced in combination with existing growth promoters such as insulin, transferrin, and selenium. Overall, the rearing pellet hydrolysates were most effective in promoting growth particularly when in combination with the growth promoters. Our findings suggest that leveraging these materials, along with known growth factors, can facilitate the development of improved, scalable, and commercially viable media for cellular agriculture.
细胞农业是一种新兴技术，旨在通过可扩展的组织培养方法生产肉类等动物制品。传统技术依赖于使用胎牛血清（FBS）的化学成分未定义的培养基或使用特定生长因子的化学成分定义的培养基。为了成为传统肉类生产的可行替代品，细胞农业需要具有成本效益的材料，并建立用于生长培养基的供应链。在这里，我们研究了从百喜草、紫花苜蓿草和牛饲养颗粒中提取的水解产物。我们确定了在含有 0.1%和 0%血清的培养基中促进 C2C12 成肌细胞生长的条件。这些作用在与胰岛素、转铁蛋白和硒等现有生长促进剂联合使用时更为明显。总的来说，饲养颗粒水解产物在促进生长方面最为有效，特别是与生长促进剂联合使用时。我们的研究结果表明，利用这些材料以及已知的生长因子，可以促进为细胞农业开发改良的、可扩展的和商业上可行的培养基。
