School of Bioengineering and Health, State Key Laboratory of New Textile Materials and Advanced Processing Technologies, Wuhan Textile University, Wuhan 430200, PR China; School of Ecology and Environmental, Tibet University, Lhasa 850000, PR China; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
School of Ecology and Environmental, Tibet University, Lhasa 850000, PR China.
Food Chem. 2024 Dec 1;460(Pt 2):140625. doi: 10.1016/j.foodchem.2024.140625. Epub 2024 Jul 26.
Tert-butylhydroquinone (TBHQ) is easily overused or illegally added to edible oil and attracts a growing concern because of its cytotoxic, liver-damaging, and carcinogenic effects. Thus, a sensitive and intelligent point-of-care testing (iPOCT) method is developed to fulfill the on-site monitoring. This iPOCT method depended on a fluorescent immunochromatographic assay within 15 min. Under optimization, the limit of quantification (LOQ) was calculated as 0.03 μg mL-1. The iPOCT method provided a low limit of detection (LOD) of 0.02 μg mL, a wide linear range of 0.03-100 μg mL, and great selectivity. Recoveries by the spiking experiments ranged from 97.4% to 103.5% with relative standard deviations (RSDs) of 2.4%-4.9% in soybean, peanut, rapeseed, and corn oil samples. The results showed that the iPOCT method is highly consistent with the high-performance liquid chromatography (HPLC) method.
叔丁基对苯二酚 (TBHQ) 很容易被过度使用或非法添加到食用油中，因其细胞毒性、肝损伤和致癌作用而引起越来越多的关注。因此，开发了一种灵敏智能的即时检测（iPOCT）方法来实现现场监测。该 iPOCT 方法在 15 分钟内依赖荧光免疫层析分析。经过优化，定量限（LOQ）计算为 0.03μgmL-1。iPOCT 方法的检测限（LOD）低至 0.02μgmL-1，线性范围宽达 0.03-100μgmL-1，具有很好的选择性。在大豆、花生、油菜籽和玉米油样品中的加标实验回收率在 97.4%至 103.5%之间，相对标准偏差（RSD）为 2.4%-4.9%。结果表明，iPOCT 方法与高效液相色谱（HPLC）方法高度一致。
