Institute of Food Safety and Nutrition, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Institute of Food Safety and Nutrition, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Microb Pathog. 2024 Sep;194:106832. doi: 10.1016/j.micpath.2024.106832. Epub 2024 Jul 31.
Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) and Enterotoxigenic E. coli (ETEC) have been found to readily develop biofilms on cucumber (Cucumis sativus L.), presenting a significant risk to the safety of ready-to-eat vegetables. This study aimed to assess the effectiveness of the lytic bacteriophage vB_EcoM_SQ17 (SQ17) against EHEC O157:H7 and ETEC biofilms on cucumber. Here, we evaluated the efficacy of phage SQ17 on the formation and reduction of biofilms formed by EHEC O157:H7 and ETEC strains on various surfaces, including polystyrene, poly-d-lysine precoated films, and fresh-cut cucumber, at different temperatures. Phage SQ17 significantly inhibited ETEC biofilm formation, reducing the number of adhered cells by 0.15 log CFU/mL at 37 °C. Treatment with phage SQ17 also significantly decreased the number of adhered cells in established biofilms via SEM observation. Moreover, phage SQ17 effectively reduced the biomass of EHEC O157:H7 and ETEC biofilms by over 54.8 % at 37 °C after 24 h of incubation. Following phage treatment, the viability of adhered EHEC O157:H7 cells decreased by 1.37 log CFU/piece and 0.46 log CFU/piece in biofilms on cucumber at 4 °C and 25 °C, respectively. Similarly, the viability of ETEC cells decreased by 1.07 log CFU/piece and 0.61 log CFU/piece in biofilms on cucumber at 4 °C and 25 °C, respectively. These findings suggest that phage SQ17 shows promise as a potential strategy for eradicating pathogenic E. coli biofilms on cucumber.
肠出血性大肠杆菌 O157:H7（EHEC O157:H7）和肠致病性大肠杆菌（ETEC）已被发现可在黄瓜（Cucumis sativus L.）上迅速形成生物膜，这对即食蔬菜的安全构成了重大威胁。本研究旨在评估裂解噬菌体 vB_EcoM_SQ17（SQ17）对黄瓜上 EHEC O157:H7 和 ETEC 生物膜的有效性。在这里，我们评估了噬菌体 SQ17 在不同温度下对 EHEC O157:H7 和 ETEC 菌株在聚苯乙烯、聚多赖氨酸预涂膜和新鲜切黄瓜等各种表面形成和减少生物膜的功效。噬菌体 SQ17 显著抑制了 ETEC 生物膜的形成，在 37°C 时将附着细胞的数量减少了 0.15 对数 CFU/mL。通过 SEM 观察，噬菌体 SQ17 处理还显著减少了已建立的生物膜中的附着细胞数量。此外，噬菌体 SQ17 在 37°C 孵育 24 小时后，可有效减少 EHEC O157:H7 和 ETEC 生物膜的生物量，超过 54.8%。噬菌体处理后，附着在黄瓜上的 EHEC O157:H7 细胞在 4°C 和 25°C 下的生物膜中的存活率分别降低了 1.37 对数 CFU/片和 0.46 对数 CFU/片。同样，ETEC 细胞在 4°C 和 25°C 下的生物膜中的存活率分别降低了 1.07 对数 CFU/片和 0.61 对数 CFU/片。这些发现表明，噬菌体 SQ17 有望成为消除黄瓜上致病性大肠杆菌生物膜的潜在策略。
