Sadekuzzaman Mohammad, Yang Sungdae, Mizan Md Furkanur Rahaman, Ha Sang-Do
School of Food Science and Technology, Chung-Ang Univ., 72-1 Nae-Ri, Daedeok-Myun, Anseong, Gyunggido, 456-756, South Korea.
Dept. of Livestock Services, People's Republic of Bangladesh.
J Food Sci. 2017 Jun;82(6):1433-1442. doi: 10.1111/1750-3841.13729. Epub 2017 May 19.
Biofilm formation is a growing concern in the food industry. Escherichia coli O157:H7 is one of the most important foodborne pathogens that can persists in food and food-related environments and subsequently produce biofilms. The efficacy of bacteriophage BPECO 19 was evaluated against three E. coli O157:H7 strains in biofilms. Biofilms of the three E. coli O157:H7 strains were grown on abiotic (stainless steel, rubber, and minimum biofilm eradication concentration [MBEC ] device) and biotic (lettuce) surfaces at different temperatures. The effectiveness of bacteriophage BPECO 19 in reducing preformed biofilms on these surfaces was further evaluated by treating the surfaces with a phage suspension (10 PFU/mL) for 2 h. The results indicated that the phage treatment significantly reduced (P < 0.05) the number of adhered cells in all the surfaces. Following phage treatment, the viability of adhered cells was reduced by ≥3 log CFU/cm , 2.4 log CFU/cm , and 3.1 log CFU/peg in biofilms grown on stainless steel, rubber, and the MBEC device, respectively. Likewise, the phage treatment reduced cell viability by ≥2 log CFU/cm in biofilms grown on lettuce. Overall, these results suggested that bacteriophages such as BPECO 19 could be effective in reducing the viability of biofilm-adhered cells.
生物膜形成是食品工业中日益受到关注的问题。大肠杆菌O157:H7是最重要的食源性病原体之一，它能够在食品及与食品相关的环境中存活，并随后形成生物膜。对噬菌体BPECO 19针对生物膜中三种大肠杆菌O157:H7菌株的效力进行了评估。三种大肠杆菌O157:H7菌株的生物膜在不同温度下于非生物（不锈钢、橡胶和最低生物膜根除浓度[MBEC]装置）和生物（生菜）表面形成。通过用噬菌体悬液（10 PFU/mL）处理这些表面2小时，进一步评估了噬菌体BPECO 19在减少这些表面上预先形成的生物膜方面的有效性。结果表明，噬菌体处理显著减少了（P < 0.05）所有表面上附着细胞的数量。噬菌体处理后，在不锈钢、橡胶和MBEC装置上生长的生物膜中，附着细胞的活力分别降低了≥3 log CFU/cm²、2.4 log CFU/cm²和3.1 log CFU/栓。同样，噬菌体处理使生菜上生长的生物膜中的细胞活力降低了≥2 log CFU/cm²。总体而言，这些结果表明，诸如BPECO 19之类的噬菌体在降低生物膜附着细胞的活力方面可能是有效的。
