Anhui Provincial Key Laboratory for Quality Evaluation and Improvement of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Gene. 2024 Nov 30;928:148810. doi: 10.1016/j.gene.2024.148810. Epub 2024 Jul 31.
Caffeoyl-coenzyme 3 A-O-methyltransferase (CCoAOMT) plays a crucial role in the lignin synthesis in many higher plants. In this study, nine PbCCoAOMT genes in total were identified from pear, and classified into six categories. We treated pear fruits with hormones abscisic acid (ABA) and methyl jasmonate (MeJA) and salicylic acid (SA) and observed differential expression levels of these genes. Through qRT-PCR, we also preliminarily identified candidate PbCCoAOMT gene, potentially involved in lignin synthesis in pear fruits. Additionally, the overexpression of PbCCoAOMT1/2 in Arabidopsis and pear fruits increased in lignin content. Enzymatic assays showed that recombinant PbCCoAOMT1/2 proteins have similar enzymatic activity in vitro. The Y1H (Yeast one-hybrid) and dual luciferase (dual-LUC) experiments demonstrated that PbMYB25 can bind to the AC elements in the promoter region of the PbCCoAOMT1 gene. Our findings suggested that the PbCCoAOMT1 and PbCCoAOMT2 genes may contribute to the synthesis of lignin and provide insights into the mechanism of lignin biosynthesis and stone cell development in pear fruits.
咖啡酰辅酶 A-O-甲基转移酶（CCoAOMT）在许多高等植物的木质素合成中起着至关重要的作用。在这项研究中，从梨中共鉴定出 9 个 PbCCoAOMT 基因，并将其分为 6 类。我们用激素脱落酸（ABA）、茉莉酸甲酯（MeJA）和水杨酸（SA）处理梨果实，观察这些基因的差异表达水平。通过 qRT-PCR，我们还初步鉴定出候选 PbCCoAOMT 基因，该基因可能参与梨果实的木质素合成。此外，PbCCoAOMT1/2 在拟南芥和梨果实中的过表达增加了木质素的含量。酶活性测定表明，重组 PbCCoAOMT1/2 蛋白在体外具有相似的酶活性。Y1H（酵母单杂交）和双荧光素酶（dual-LUC）实验表明，PbMYB25 可以与 PbCCoAOMT1 基因启动子区的 AC 元件结合。我们的研究结果表明，PbCCoAOMT1 和 PbCCoAOMT2 基因可能有助于木质素的合成，并为梨果实木质素生物合成和石细胞发育的机制提供了新的见解。
