3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal; BIOtech Research Center, Department of Industrial Engineering, University of Trento, Via Delle Regole 101, 38123 Trento, Italy.
3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Int J Biol Macromol. 2024 Oct;277(Pt 4):134250. doi: 10.1016/j.ijbiomac.2024.134250. Epub 2024 Jul 30.
The current treatments for wounds often fail to induce adequate healing, leaving wounds vulnerable to persistent infections and development of drug-resistant microbial biofilms. New natural-derived nanoparticles were studied to impair bacteria colonization and hinder the formation of biofilms in wounds. The nanoparticles were fabricated through polyelectrolyte complexation of chitosan (CS, polycation) and hyaluronic acid (HA, polyanion). UV-induced photo-crosslinking was used to enhance the stability of the nanoparticles. To achieve this, HA was methacrylated (HAMA, degree of modification of 20 %). Photo-crosslinked nanoparticles obtained from HAMA and CS had a diameter of 478 nm and a more homogeneous size distribution than nanoparticles assembled solely through complexation (742 nm). The nanoparticles were loaded with the antimicrobial agent bacitracin (BC), resulting in nanoparticles with a diameter of 332 nm. The encapsulation of BC was highly efficient (97 %). The BC-loaded nanoparticles showed significant antibacterial activity against gram-positive bacteria Staphylococcus aureus, Methicillin-resistant S. aureus and S. epidermidis. Photo-crosslinked HAMA/CS nanoparticles loaded with BC demonstrated inhibition of biofilm formation and a positive effect on the proliferation of mammalian cells (L929). These crosslinked nanoparticles have potential for the long-term treatment of wounds and controlled antibiotic delivery at the location of a lesion.
目前的伤口治疗方法往往无法诱导充分的愈合，使伤口容易受到持续感染和耐药微生物生物膜的形成。新的天然衍生纳米粒子被研究用于破坏细菌定植并阻碍伤口中生物膜的形成。纳米粒子是通过壳聚糖（CS，聚阳离子）和透明质酸（HA，聚阴离子）的聚电解质复合形成的。使用紫外光诱导的光交联来增强纳米粒子的稳定性。为此，HA 被甲基丙烯酰化（HAMA，修饰度为 20%）。与仅通过复合物组装的纳米粒子（742nm）相比，从 HAMA 和 CS 获得的光交联纳米粒子的直径为 478nm，且具有更均匀的粒径分布。将抗菌剂杆菌肽（BC）负载到纳米粒子上，得到直径为 332nm 的纳米粒子。BC 的包封效率非常高（97%）。载有 BC 的纳米粒子对革兰氏阳性菌金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和表皮葡萄球菌表现出显著的抗菌活性。负载 BC 的光交联 HAMA/CS 纳米粒子抑制生物膜形成，并对哺乳动物细胞（L929）的增殖有积极影响。这些交联纳米粒子具有在病变部位长期治疗伤口和控制抗生素递送的潜力。
