College of Food Science and Engineering, Hunan Key Laboratory of Processed Food for Special Medical Purpose, Central South University of Forestry and Technology, Changsha 410004, China.
Xiang Ya School of Public Health, Central South University, Changsha 410078, China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134309. doi: 10.1016/j.ijbiomac.2024.134309. Epub 2024 Jul 31.
Plant-derived exosome-like nanovesicles (ELNs) are nano-sized vesicles extracted from edible plants. Lycium ruthenicum Murray (LRM) has been gaining increasing attention due to its nutritional and medicinal value, but the ELNs in LRM has not been reported. In this study, LRM-ELNs were obtained, and the proteins, lipids, microRNAs (miRNAs) and active components in LRM tissues and LRM-ELNs was analyzed by LC-MS/MS, LC-MS, high-throughput sequencing techniques, and physical and chemical analysis. LRM-ELNs can be uptaken by PC12 cells through macropinocytosis and caveolin-mediated endocytosis primarily. Transcriptomic and western blot experiments indicate that LRM-ELNs can inhibit Aβ-induced apoptosis in PC12 cells through the MAPK and PI3K/AKT signaling pathways, with miRNAs playing a crucial role. These results indicate that LRM-ELNs have the protection effect on PC12 cells and can be considered as dietary supplements for alleviating neurodegenerative diseases.
植物来源的外泌体样纳米囊泡（ELNs）是从可食用植物中提取的纳米大小的囊泡。黑果枸杞（LRM）由于其营养价值和药用价值而受到越来越多的关注，但 LRM 中的 ELNs 尚未被报道。在本研究中，获得了 LRM-ELNs，并通过 LC-MS/MS、LC-MS、高通量测序技术以及物理和化学分析，分析了 LRM 组织和 LRM-ELNs 中的蛋白质、脂质、microRNAs（miRNAs）和活性成分。LRM-ELNs 可以通过巨胞饮作用和网格蛋白介导的内吞作用主要被 PC12 细胞摄取。转录组和 Western blot 实验表明，LRM-ELNs 通过 MAPK 和 PI3K/AKT 信号通路抑制 Aβ诱导的 PC12 细胞凋亡，miRNAs 在此过程中发挥关键作用。这些结果表明 LRM-ELNs 对 PC12 细胞具有保护作用，可作为缓解神经退行性疾病的膳食补充剂。
