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一种带有苯基硼酸单元的类黑色素聚合物，作为用于活细胞展示技术的合适生物平台。

A melanin-like polymer bearing phenylboronic units as a suitable bioplatform for living cell display technology.

作者信息

Vona Danilo, Cicco Stefania Roberta, Vicente-Garcia Cesar, Digregorio Alessandro, Rizzo Giorgio, Labarile Rossella, Giangregorio Maria Michela, Porfido Carlo, Terzano Roberto, Altamura Emiliano, Cotugno Pietro, Farinola Gianluca Maria

机构信息

Dipartimento Di Scienze del Suolo, Della Pianta E Degli Alimenti (Di.S.S.P.A.), Università Degli Studi Di Bari "Aldo Moro", Via G. Amendola 165/A, 70126, Bari, Italy.

Consiglio Nazionale Delle Ricerche, CNR-ICCOM, Via E. Orabona 4, 70126, Bari, Italy.

出版信息

Sci Rep. 2024 Aug 1;14(1):17856. doi: 10.1038/s41598-024-68932-4.

DOI:10.1038/s41598-024-68932-4
PMID:39090178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11294599/
Abstract

Surface display of functional groups with specific reactivity around living cells is an emerging, low cost and highly eco-compatible technology that serves multiple applications, ranging from basic biochemical studies to biomedicine, therapeutics and environmental sciences. Conversely to classical methods exploiting hazardous organic synthesis of precursors or monovalent functionalization via genetics, here we perform functional decoration of individual living microalgae using suitable biocoatings based on polydopamine, a melanin-like synthetic polymer. Here we demonstrate the one-pot synthesis of a functional polydopamine bearing phenylboronic units which can decorate the living cell surfaces via a direct ester formation between boronic units and surface glycoproteins. Furthermore, biosorption of fluorescent sugars on functionalized cell membranes is triggered, demonstrating that these organic coatings act as biocompatible soft shells, still functional and reactive after cell engineering.

摘要

在活细胞周围展示具有特定反应性的官能团是一项新兴的、低成本且高度生态兼容的技术，可用于多种应用，从基础生化研究到生物医学、治疗学和环境科学。与利用危险的前体有机合成或通过遗传学进行单价功能化的经典方法相反，在这里，我们使用基于聚多巴胺（一种类黑色素合成聚合物）的合适生物涂层对单个活微藻进行功能修饰。在这里，我们展示了一锅法合成带有苯基硼酸单元的功能性聚多巴胺，该聚多巴胺可通过硼酸单元与表面糖蛋白之间的直接酯形成来修饰活细胞表面。此外，触发了荧光糖在功能化细胞膜上的生物吸附，表明这些有机涂层充当生物相容的软壳，在细胞工程后仍具有功能和反应性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/f7135ee45351/41598_2024_68932_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/6b6aafaaf368/41598_2024_68932_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/e02c0d3dc727/41598_2024_68932_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/ac75648f3604/41598_2024_68932_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/3cad9c242b97/41598_2024_68932_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/fb05616b2453/41598_2024_68932_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/f7135ee45351/41598_2024_68932_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/6b6aafaaf368/41598_2024_68932_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/e02c0d3dc727/41598_2024_68932_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/ac75648f3604/41598_2024_68932_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/3cad9c242b97/41598_2024_68932_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/fb05616b2453/41598_2024_68932_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1087/11294599/f7135ee45351/41598_2024_68932_Fig6_HTML.jpg

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本文引用的文献

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Improving the In Vitro Removal of Indoxyl Sulfate and p-Cresyl Sulfate by Coating Diatomaceous Earth (DE) and Poly-vinyl-pyrrolidone-co-styrene (PVP-co-S) with Polydopamine.用聚多巴胺对硅藻土（DE）和聚-乙烯基-吡咯烷酮-共-苯乙烯（PVP-co-S）进行包被，以提高其对硫酸吲哚酚和对甲酚硫酸盐的体外去除率。
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Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes.
用于完整细菌生物杂交光阳极的仿生氧化还原粘合剂聚多巴胺基质
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Incorporating a molecular antenna in diatom microalgae cells enhances photosynthesis.在硅藻微藻细胞中加入分子天线可增强光合作用。
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