Department of Chemistry, Università degli Studi "Aldo Moro", Via Orabona 4, 70126, Bari, Italy.
Department of Chemistry, CNR-ICCOM-Bari, Via Orabona 4, 70126, Bari, Italy.
Photochem Photobiol Sci. 2022 Jun;21(6):949-958. doi: 10.1007/s43630-022-00185-4. Epub 2022 Feb 14.
Many microorganisms produce specific structures, known as spores or cysts, to increase their resistance to adverse environmental conditions. Scientists have started to produce biomimetic materials inspired by these natural membranes, especially for industrial and biomedical applications. Here, we present biological data on the biocompatibility of a polydopamine-based artificial coating for diatom cells. In this work, living Thalassiosira weissflogii diatom cells are coated on their surface with a polydopamine layer mimicking mussel adhesive protein. Polydopamine does not affect diatoms growth kinetics, it enhances their resistance to degradation by treatment with detergents and acids, and it decreases the uptake of model staining emitters. These outcomes pave the way for the use of living diatom cells bearing polymer coatings for sensors based on living cells, resistant to artificial microenvironments, or acting as living devices for cells interface study.
许多微生物会产生特定的结构，称为孢子或芽孢，以提高它们对不利环境条件的抵抗力。科学家们已经开始受这些天然膜启发来制造仿生材料，特别是用于工业和生物医学应用。在这里，我们提供了基于多巴胺的人工涂层对硅藻细胞生物相容性的生物学数据。在这项工作中，活的威氏海链藻硅藻细胞表面涂有一层模拟贻贝类黏附蛋白的多巴胺层。多巴胺不会影响硅藻的生长动力学，它增强了它们对去污剂和酸处理的降解的抵抗力，并减少了模型染色发射体的摄取。这些结果为使用带有聚合物涂层的活硅藻细胞为基于活细胞的传感器铺平了道路，这些传感器能够抵抗人工微环境，或者作为细胞界面研究的活体装置。
