Wang Lei, Nabi Fazul, Zhang Xiaohu, Zhou Guangyu, Shah Qurban Ali, Li Siyuan, Lu Yaozhong, Mu Siyang, Zhu Xiaohui, Lin Zhengrong, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, 90150, Pakistan.
Probiotics Antimicrob Proteins. 2024 Aug 1. doi: 10.1007/s12602-024-10336-x.
Given China's prohibition on the utilization of antibiotics as feed additives in 2020, we aim to investigate nutrition additives that are both efficient and safe. Lactobacillus, a well-recognized beneficial probiotic, has explicitly been investigated for its effects on health status of the host and overall impact on food industry. To evaluate effects of Lactobacillus plantarum (LW) supplementation on broiler chicken, we conducted comprehensive multi-omics analysis, growth performance evaluation, RT-qPCR analysis, and immunofluorescence. The findings revealed that LW supplementation resulted in a substantial progress in growth performance (approximately 205 g increase in final body weight in comparison to the control group (p < 0.01)). Additionally, LW exhibited promising potential for enhancing antioxidant properties of serum and promoting gut integrity and growth as evidenced by improved antioxidant indices (p < 0.01), intestinal villus morphology (p < 0.01), and enhanced gut barrier function (p < 0.01). Meanwhile, the multi-omics analysis, including 16S rRNA sequencing and liquid chromatography-tandem mass spectrometry, revealed an enrichment of beneficial microbes in the gut of broilers that were supplemented with LW, while simultaneously depleting harmful microorganisms. Moreover, a noteworthy modification was observed in gut metabolic profiling subsequent to the execution of the probiotic strategy. Specifically, variations were noticed in the levels of metabolites and metabolic pathways such as parathyroid hormone synthesis, inflammatory mediator regulation of TRP channels, oxidative phosphorylation, and mineral absorption. Taken together, our findings validate that LW administration produces valuable effects on the health and growth performance of broilers owing to its capability to boost the gut microbiota homeostasis and intestinal metabolism. Present findings signify the potential of LW as a dietary additive to promote growth and development in broiler chickens.
鉴于中国在2020年禁止将抗生素用作饲料添加剂，我们旨在研究既高效又安全的营养添加剂。乳酸杆菌是一种公认的有益益生菌，已对其对宿主健康状况的影响以及对食品工业的总体影响进行了明确研究。为了评估植物乳杆菌（LW）添加对肉鸡的影响，我们进行了全面的多组学分析、生长性能评估、RT-qPCR分析和免疫荧光分析。研究结果表明，添加LW使生长性能有了显著提高（与对照组相比，最终体重增加了约205克，p < 0.01）。此外，LW在增强血清抗氧化性能、促进肠道完整性和生长方面显示出有前景的潜力，抗氧化指标改善（p < 0.01）、肠绒毛形态改善（p < 0.01）以及肠道屏障功能增强（p < 0.01）都证明了这一点。同时，包括16S rRNA测序和液相色谱-串联质谱在内的多组学分析表明，添加LW的肉鸡肠道中有益微生物富集，同时有害微生物减少。此外，在实施益生菌策略后，肠道代谢谱发生了值得注意的变化。具体而言，在甲状旁腺激素合成、TRP通道的炎症介质调节、氧化磷酸化和矿物质吸收等代谢物水平和代谢途径中发现了变化。综上所述，我们的研究结果证实，由于LW能够促进肠道微生物群稳态和肠道代谢，其对肉鸡的健康和生长性能产生了有益影响。目前的研究结果表明LW作为一种膳食添加剂在促进肉鸡生长发育方面的潜力。
