Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Belarusian State University, Minsk, Republic of Belarus.
Bull Exp Biol Med. 2024 Jun;177(2):197-202. doi: 10.1007/s10517-024-06155-3. Epub 2024 Aug 2.
Type 2 diabetes mellitus (T2DM) is accompanied by halogenative stress resulting from the excessive activation of neutrophils and neutrophilic myeloperoxidase (MPO) generating highly reactive hypochlorous acid (HOCl). HOCl in blood plasma modifies serum albumin (Cl-HSA). We studied the formation of neutrophil extracellular traps (NETs) in the whole blood and by isolated neutrophils under the action of Cl-HSA. It was found that Cl-HSA induces neutrophil priming and NETosis. MPO-containing as well as MPO-free NETs were found. These NETs with different composition can be a product of NETosis of one and the same neutrophil. NET formation in neutrophils with vacuolated cytoplasm was detected. In the presence of Cl-HSA, acceleration of NET degradation was observed. Accelerated NET degradation and neutrophil priming can be the factors contributing to the development of complications in T2DM.
2 型糖尿病（T2DM）伴随着由中性粒细胞过度激活和髓过氧化物酶（MPO）产生的卤化应激，生成高反应性次氯酸（HOCl）。HOCl 在血浆中修饰血清白蛋白（Cl-HSA）。我们研究了 Cl-HSA 在全血中和分离的中性粒细胞中的作用下形成中性粒细胞胞外诱捕网（NETs）的情况。结果发现，Cl-HSA 诱导中性粒细胞的预激活和 NETosis。发现含有 MPO 和无 MPO 的 NETs。这些组成不同的 NETs可能是同一中性粒细胞 NETosis 的产物。在含有空泡细胞质的中性粒细胞中检测到 NET 形成。在 Cl-HSA 的存在下，观察到 NET 降解的加速。加速的 NET 降解和中性粒细胞预激活可能是 T2DM 并发症发展的因素。
