Shmeleva E V, Basyreva L Yu, Vakhrusheva T V, Sokolov A V, Gusev S A, Panasenko O M, Sergienko V I
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Institute of Experimental Medicine, St. Petersburg, Russia.
Bull Exp Biol Med. 2025 Aug 29. doi: 10.1007/s10517-025-06473-0.
Extracellular traps (ETs) released during ETosis of various blood cells not only participate in host defense against pathogenic microorganisms, but also play an essential role in diverse pathological processes. It should be noted that ETs are heterogeneous by their structure; therefore, for studying their pathophysiological effects, it is extremely important to create model ETs with strictly controlled composition and structure. We propose an approach that allows creating model mesh structures based on DNA and histones (DNA-histone networks, DHN), which can be considered as model ETs. It has been shown that the structure of DHN depends on the DNA/histone weight ratio. The possibility of incorporating myeloperoxidase that can be present in native ETs, into DHN has been demonstrated. The creation of DHN with controlled composition and structure will presumably allow assessing the specificity of enzyme systems involved in the degradation of native ETs. In addition, these heterogeneous DHN can be used to study the relationship between ET structure and ET-induced thrombosis or their antimicrobial activity.
各种血细胞在胞外诱捕作用过程中释放的细胞外陷阱（ETs）不仅参与宿主对病原微生物的防御，还在多种病理过程中发挥重要作用。需要注意的是，ETs的结构具有异质性；因此，为了研究它们的病理生理效应，创建具有严格可控组成和结构的模型ETs极其重要。我们提出了一种方法，该方法能够基于DNA和组蛋白创建模型网状结构（DNA-组蛋白网络，DHN），其可被视为模型ETs。研究表明，DHN的结构取决于DNA/组蛋白的重量比。已经证实了将天然ETs中可能存在的髓过氧化物酶纳入DHN的可能性。创建具有可控组成和结构的DHN大概将有助于评估参与天然ETs降解的酶系统的特异性。此外，这些异质性的DHN可用于研究ET结构与ET诱导的血栓形成或其抗菌活性之间的关系。
