大肠杆菌16S rRNA 3' 小结构域中的点突变
Point mutations in the 3' minor domain of 16S rRNA of E.coli.
作者信息
Jemiolo D K, Zwieb C, Dahlberg A E
出版信息
Nucleic Acids Res. 1985 Dec 9;13(23):8631-43. doi: 10.1093/nar/13.23.8631.
Abstract
Point mutations were produced near the 3' end of E. coli 16S rRNA by bisulfite mutagenesis in a 121 base loop-out (1385 to 1505) in a heteroduplex of wild type (pKK3535) and deletion mutant plasmids. Two highly conserved, single stranded regions flank an irregular helix (1409-1491) in the area studied. Only a single mutation was isolated in the flanking regions, a transition at C1402, (normally methylated on the base and ribose in rRNA). Mutations occurred throughout the irregular helix. All mutant rRNAs were processed and assembled into 30S subunits capable of interacting with 50S subunits. Growth rates ranged from faster to significantly slower than cells with the wild type transcript. In particular, mutations at C1467 or C1469 cause slow growth. These two transitions (in a bulge region within the helix) reduced the bulge by additional base pairing.
摘要
通过亚硫酸氢盐诱变，在野生型（pKK3535）和缺失突变体质粒的异源双链体中，于大肠杆菌16S rRNA的3'端附近、121个碱基的环出区域（1385至1505）产生了点突变。在所研究区域，两个高度保守的单链区域位于一个不规则螺旋（1409 - 1491）两侧。侧翼区域仅分离到一个突变，即C1402处的转换（rRNA中该碱基和核糖通常被甲基化）。突变发生在整个不规则螺旋区域。所有突变的rRNA都经过加工并组装成能够与50S亚基相互作用的30S亚基。生长速率范围从比具有野生型转录本的细胞快到显著慢。特别是，C1467或C1469处的突变导致生长缓慢。这两个转换（在螺旋内的一个凸起区域）通过额外的碱基配对减少了凸起。
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