Shine J, Dalgarno L
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6. doi: 10.1073/pnas.71.4.1342.
With a stepwise degradation and terminal labeling procedure the 3'-terminal sequence of E. coli 16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-A(OH). It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons. The sequence A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus be involved in the formation of the mRNA.30S subunit complex.
通过逐步降解和末端标记程序,大肠杆菌16S核糖体RNA的3'-末端序列显示为Pyd-A-C-C-U-C-C-U-U-A(OH)。有人提出,RNA的这一区域能够与mRNA相互作用,并且3'-末端的U-U-A(OH)通过与终止密码子碱基配对参与蛋白质合成的终止。序列A-C-C-U-C-C可以识别各种大肠杆菌噬菌体mRNA核糖体结合位点中发现的确保守序列;因此它可能参与mRNA-30S亚基复合物的形成。