Zhou Xue-Hai, Hua Min-Min, Tang Jia-Nan, Wu Bang-Guo, Wang Xue-Mei, Shi Chang-Gen, Yang Yang, Wu Jun, Wu Bin, Zhang Bao-Li, Sun Yi-Si, Zhang Tian-Cheng, Shi Hui-Juan
Shanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies, School of Pharmacy, Fudan University, Shanghai 200237, China.
Asian J Androl. 2025 Jan 1;27(1):120-128. doi: 10.4103/aja202442. Epub 2024 Aug 2.
The genome tagging project (GTP) plays a pivotal role in addressing a critical gap in the understanding of protein functions. Within this framework, we successfully generated a human influenza hemagglutinin-tagged sperm-specific protein 411 (HA-tagged Ssp411) mouse model. This model is instrumental in probing the expression and function of Ssp411. Our research revealed that Ssp411 is expressed in the round spermatids, elongating spermatids, elongated spermatids, and epididymal spermatozoa. The comprehensive examination of the distribution of Ssp411 in these germ cells offers new perspectives on its involvement in spermiogenesis. Nevertheless, rigorous further inquiry is imperative to elucidate the precise mechanistic underpinnings of these functions. Ssp411 is not detectable in metaphase II (MII) oocytes, zygotes, or 2-cell stage embryos, highlighting its intricate role in early embryonic development. These findings not only advance our understanding of the role of Ssp411 in reproductive physiology but also significantly contribute to the overarching goals of the GTP, fostering groundbreaking advancements in the fields of spermiogenesis and reproductive biology.
基因组标记项目（GTP）在弥补蛋白质功能理解方面的关键差距中发挥着核心作用。在此框架内，我们成功构建了一种人流感血凝素标记的精子特异性蛋白411（HA标记的Ssp411）小鼠模型。该模型有助于探究Ssp411的表达和功能。我们的研究表明，Ssp411在圆形精子细胞、伸长精子细胞、成熟精子细胞和附睾精子中均有表达。对Ssp411在这些生殖细胞中的分布进行全面研究，为其参与精子发生提供了新的视角。然而，必须进行严格的进一步研究，以阐明这些功能的确切机制基础。在中期II（MII）卵母细胞、受精卵或2细胞期胚胎中未检测到Ssp411，这突出了其在早期胚胎发育中的复杂作用。这些发现不仅增进了我们对Ssp411在生殖生理学中作用的理解，也为GTP的总体目标做出了重大贡献，推动了精子发生和生殖生物学领域的突破性进展。
