School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, China.
Institute of Technology for Chinese Medicine Preparations Industry, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, China.
J Sep Sci. 2024 Aug;47(15):e2400275. doi: 10.1002/jssc.202400275.
In this study, we propose a novel strategy utilizing deep eutectic solvents (DESs) as both the extraction solvent and dispersing liquid, with nanometer zinc oxide (ZnO) serving as the adsorbent. This method incorporates ultrasound-assisted matrix solid phase dispersion (UA-MSPD) for the extraction of six active components (salidroside, echinacoside, acteoside, specnuezhenide, nuezhenoside G13, and oleanolic acid) from Ligustri Lucidi Fructus samples. The extracts were then analyzed using high-performance liquid chromatography equipped with a diode array detector. The effects of various parameters such as dispersant dosage, DESs volume, grinding time, ultrasonication duration, and eluent volume on extraction recovery were investigated and optimized using a central composite design under response surface methodology. The optimized conditions yielded detection limits ranging from 0.003 to 0.01 mg/g and relative standard deviations of 8.7% or lower. Extraction recoveries varied between 93% and 98%. The method demonstrated excellent linearity for the analytes (R ≥ 0.9997). The simple, green, and efficient DESs/ZnO-UA-MSPD technique proved to be rapid, accurate, and reliable for extracting and analyzing the six active ingredients in Ligustri Lucidi Fructus samples.
在本研究中，我们提出了一种新策略，利用深共晶溶剂 (DESs) 作为提取溶剂和分散液，纳米氧化锌 (ZnO) 作为吸附剂。该方法结合超声辅助基质固相分散 (UA-MSPD) 从女贞子样品中提取六种活性成分（红景天苷、毛蕊花糖苷、麦角甾苷、松果菊苷、新女贞苷 G13 和齐墩果酸）。然后使用配备二极管阵列检测器的高效液相色谱法对提取物进行分析。通过响应面法中的中心复合设计，研究并优化了各种参数（分散剂用量、DESs 体积、研磨时间、超声时间和洗脱剂体积）对提取回收率的影响。在优化条件下，检测限范围为 0.003 至 0.01mg/g，相对标准偏差低于 8.7%。提取回收率在 93%至 98%之间。该方法对分析物具有良好的线性（R≥0.9997）。简单、绿色、高效的 DESs/ZnO-UA-MSPD 技术快速、准确、可靠，可用于提取和分析女贞子样品中的六种活性成分。
