Nambiar Dhanya, Le Quynh-Thu, Pucci Ferdinando
Department of Radiation Oncology, Stanford University, Stanford, CA, United States.
Otolaryngology Department, Head and Neck Surgery, Oregon Health & Science University, Portland, OR, United States.
Front Oncol. 2024 Jul 12;14:1430971. doi: 10.3389/fonc.2024.1430971. eCollection 2024.
Three main areas of research revolve around extracellular vesicles (EVs): their use as early detection diagnostics for cancer prevention, engineering of EVs or other enveloped viral-like particles for therapeutic purposes and to understand how EVs impact biological processes. When investigating the biology of EVs, it is important to consider strategies able to track and alter EVs directly , as they are released by donor cells. This can be achieved by suitable engineering of EV donor cells, either before implantation or directly Here, we make a case for the study of native EVs, that is, EVs released by cells living within a tissue. Novel genetic approaches to detect intercellular communications mediated by native EVs and profile recipient cells are discussed. The use of Rab35 dominant negative mutant is proposed for functional studies on the roles of native EVs. Ultimately, investigations on native EVs will tremendously advance our understanding of EV biology and open novel opportunities for therapy and prevention.
细胞外囊泡（EVs）的研究主要围绕三个主要领域展开：将其用作癌症预防的早期检测诊断工具；对EVs或其他包膜病毒样颗粒进行工程改造以用于治疗目的；以及了解EVs如何影响生物过程。在研究EVs的生物学特性时，考虑能够直接追踪和改变由供体细胞释放的EVs的策略非常重要。这可以通过在植入前或直接对EV供体细胞进行适当的工程改造来实现。在这里，我们主张研究天然EVs，即由组织内的细胞释放的EVs。讨论了检测由天然EVs介导的细胞间通讯并分析受体细胞的新型遗传方法。建议使用Rab35显性负性突变体对天然EVs的作用进行功能研究。最终，对天然EVs的研究将极大地推进我们对EV生物学的理解，并为治疗和预防开辟新的机会。
