Ayoubi Riham, Southern Kathleen, Laflamme Carl
Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
F1000Res. 2024 Aug 1;12:148. doi: 10.12688/f1000research.130587.3. eCollection 2023.
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In this study, we characterized 8 commercial Midkine antibodies for Western blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in a knockout cell line and isogenic parental control. These studies are part of a larger, collaborative initiative seeking to address the antibody reproducibility issue by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
中期因子是一种分泌蛋白，作为生长因子或细胞因子参与细胞存活和炎症过程。它在淀粉样斑块中积累，而淀粉样斑块是阿尔茨海默病（AD）的标志。如果科学界能够获得特性明确的抗中期因子抗体，中期因子研究的可重复性将会提高。在本研究中，我们使用基于比较基因敲除细胞系和同基因亲本对照读数的标准化实验方案，对8种用于蛋白质印迹和免疫沉淀的商业化中期因子抗体进行了特性分析。这些研究是一项更大的合作计划的一部分，该计划旨在通过对市售的人源蛋白抗体进行特性分析并将结果公开发布作为科学界的资源，来解决抗体可重复性问题。虽然各实验室使用的抗体和方案各不相同，但我们鼓励读者将本报告作为指南，为其特定需求选择最合适的抗体。
