[一种同时测定乙肝表面抗原（HBsAg）和乙肝表面抗体（抗-HBs）的免疫酶法的评估]
[Evaluation of an immunoenzymatic method for the simultaneous determination of HBsAg and anti-HBs].
作者信息
Tanzi E, Perin S, De Paschale M, Cometti R, Ferroni P
出版信息
Quad Sclavo Diagn. 1985 Jun;21(2):190-8.
In this study we have evaluated an enzymeimmunoassay for the simultaneous detection of HBsAg and anti-HBs. The EIA method was found to determine anti-HBs at titer of about 10 mIU/ml and HBsAg between 1.5 and 2.5 EU/ml for both ad and ay subtypes. Detection of HBsAg and anti-HBs by EIA and RIA in sera collected from healthy subjects and in patients with acute or chronic hepatitis showed a concordance, respectively, of 98.2 and 97.4%. The discrepancies were in part due to the higher sensitivity of RIA respect to EIA method, while in some cases EIA seems to detect anti-HBs earlier than RIA. The EIA method might be effectively employed in pre-vaccine screening and in the serological follow-up of individuals who received anti-hepatitis B vaccine.
在本研究中，我们评估了一种用于同时检测HBsAg和抗-HBs的酶免疫测定法。发现该酶免疫测定法对于ad和ay亚型均可检测到效价约为10 mIU/ml的抗-HBs以及1.5至2.5 EU/ml的HBsAg。通过酶免疫测定法（EIA）和放射免疫测定法（RIA）对从健康受试者以及急慢性肝炎患者采集的血清中的HBsAg和抗-HBs进行检测，结果显示一致性分别为98.2%和97.4%。差异部分归因于放射免疫测定法相对于酶免疫测定法具有更高的灵敏度，而在某些情况下，酶免疫测定法似乎比放射免疫测定法更早检测到抗-HBs。酶免疫测定法可有效地用于疫苗接种前筛查以及接受乙肝疫苗个体的血清学随访。
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