[酶联免疫吸附测定法（ELISA）板分光光度读数在检测乙肝表面抗原/抗体系统中的优势]
[Advantages of spectrophotometric reading of ELISA plates for determination of the HBs/anti-HB system].
作者信息
Taliani G, Palombo G, Tonelli M G, Franco E
出版信息
Boll Soc Ital Biol Sper. 1981 Nov 15;57(21):2111-5.
It has been tested some diluted HBsAg and anti-HBs positive sera by modified ELISA test. We have found a correlation between HBsAg titer and spectrophotometric reading value and we have shown it in a curve. On the other hand, by neutralization of different dilutions of a RIA anti-HBs positive serum with different dilutions of a HBsAg positive serum we have carried out a checkboard and we have found that the best titer for neutralizing HBsAg is 30 ng/ml. This HBsAg has been called "standard". Using HBsAg "standard" we have tested 50 sera of hemophiliacs and we have found 1 HBsAg positive (2%) and 38 anti-HBs positive patients (76%).
已通过改良酶联免疫吸附测定法对一些稀释的乙肝表面抗原（HBsAg）和乙肝表面抗体（抗 - HBs）阳性血清进行了检测。我们发现了HBsAg滴度与分光光度读数之间的相关性，并将其绘制成曲线展示出来。另一方面，用不同稀释度的HBsAg阳性血清中和不同稀释度的放射免疫分析（RIA）抗 - HBs阳性血清，我们进行了方阵滴定试验，发现中和HBsAg的最佳滴度为30 ng/ml。这种HBsAg被称为“标准品”。使用HBsAg“标准品”检测了50例血友病患者的血清，发现1例HBsAg阳性（2%），38例抗 - HBs阳性患者（76%）。
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