Kazak Filiz, Coskun Pinar, Yarim Gul Fatma, Baspinar Nuri, Ozdemir Ozgur, Ates Mehmet Burak, Altug Muhammed Enes, Deveci Mehmet Zeki Yilmaz
Department of Biochemistry, Veterinary Faculty, Hatay Mustafa Kemal University, Hatay, Turkey.
Department of Biochemistry, Veterinary Faculty, Ondokuz Mayis University, Samsun, Turkey.
Biotech Histochem. 2024 Jul;99(5):278-285. doi: 10.1080/10520295.2024.2380663. Epub 2024 Aug 2.
Possible protective and therapeutic effects of nobiletin on kidney in a cisplatin-induced nephrotoxicity rat model were investigated. Forty male albino rats were divided into four groups: control, cisplatin (CIS), cisplatin+nobiletin (CIS+NOB), and nobiletin+cisplatin (NOB+CIS). At the end of the study, the rats were subjected to biochemical, histological and immunohistochemical analyzes. Compared to the control group, tGSH ( < 0.05) levels, and G6PD ( < 0.05) and GPx ( < 0.001) activities, were increased in the CIS group; while significant ( < 0.05) decreases occurred in the MDA and TOC levels. Histopathologically, the kidneys of the groups administered nobiletin (CIS+NOB, NOB+CIS) were significantly different from the CIS group, being closer to control group in terms of degeneration and hyaline cylinder formation in the tubules ( < 0.05). While dilatation in the tubules, protein-rich fluid and hyaline cylinder formation in the lumen were most common in the CIS group, a significant decrease ( < 0.05) of these parameters was seen in the nobiletin groups (CIS+NOB, NOB+CIS). This study suggests that nobiletin can be effective in preventing and ameliorating toxic effects of cisplatin on the kidney.
研究了诺米林对顺铂诱导的肾毒性大鼠模型肾脏可能的保护和治疗作用。将40只雄性白化大鼠分为四组：对照组、顺铂组（CIS）、顺铂+诺米林组（CIS+NOB）和诺米林+顺铂组（NOB+CIS）。在研究结束时，对大鼠进行生化、组织学和免疫组化分析。与对照组相比，CIS组的总谷胱甘肽（tGSH）水平（<0.05）、葡萄糖-6-磷酸脱氢酶（G6PD）活性（<0.05）和谷胱甘肽过氧化物酶（GPx）活性（<0.001）升高；而丙二醛（MDA）和总氧化能力（TOC）水平显著降低（<0.05）。组织病理学上，给予诺米林的组（CIS+NOB、NOB+CIS）的肾脏与CIS组有显著差异，在肾小管变性和透明管型形成方面更接近对照组（<0.05）。虽然肾小管扩张、管腔内富含蛋白质的液体和透明管型形成在CIS组最为常见，但在诺米林组（CIS+NOB、NOB+CIS）中这些参数显著降低（<0.05）。本研究表明，诺米林可有效预防和改善顺铂对肾脏的毒性作用。
