Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Moscow Region, Russia.
Bull Exp Biol Med. 2024 Jun;177(2):266-270. doi: 10.1007/s10517-024-06170-4. Epub 2024 Aug 2.
The efficiency of combinations of cytostatics cisplatin and adriamycin with antioxidant sodium 3-(3'-tert-butyl-4-hydroxyphenyl)propyl thiosulfate (TS-13), and nitric oxide (NO) donor NaNO was evaluated on two drug-resistant strains of leukemia P388 with changed redox-status of cells. Simultaneous use of both NO donor and TS-13 in combinations with the cytostatics did not increase the efficiency of therapy. In addition, antioxidant activity of TS-13, NaNO, and their combinations was studied by the method of luminol-dependent chemiluminescence on the model systems with the use of the homogenized cells of sensitive strain and two drug-resistant strains of leukemia P388. It was shown that TS-13 and NO donor produced opposite effects: TS-13 decreased, while NO donor increased the content of free radicals in the model system. Combinations of antioxidant TS-13 and NO donor should be used with consideration for the redox-status of tumor treated.
评估了细胞氧化还原状态改变的白血病 P388 两种耐药株中细胞抑制剂顺铂和阿霉素与抗氧化剂 3-(3'-叔丁基-4-羟基苯基)丙基硫代硫酸盐（TS-13）和一氧化氮（NO）供体 NaNO 的组合的效率。在细胞抑制剂联合使用两种 NO 供体和 TS-13 并没有提高治疗效率。此外，通过使用敏感株和白血病 P388 的两种耐药株的匀浆细胞的模型系统，用发光依赖性化学发光法研究了 TS-13、NaNO 及其组合的抗氧化活性。结果表明，TS-13 和 NO 供体产生相反的作用：TS-13 降低，而 NO 供体增加模型系统中自由基的含量。抗氧化剂 TS-13 和 NO 供体的组合应考虑到治疗的肿瘤的氧化还原状态来使用。
