Fan Aqing, Liu Gengliang, Wu Xiaodong
University of Science and Technology of China, Hefei, 230000, China.
Suzhou Institute of Biomedical Engineering Technology, Chinese Academy of Sciences, No. 88 Keling Road, Huqiu District, Suzhou City, 215163, Jiangsu Province, China.
J Interv Card Electrophysiol. 2024 Aug 2. doi: 10.1007/s10840-024-01828-5.
As an emerging myocardial ablation technique, the mechanism of nanosecond pulse electric field (nsPEF) ablation is currently less studied. Mitochondria are one of the important membrane structure organelles in cells, participating in numerous life activities within the cell. This study aimed to explore the morphological changes of mitochondria in living cells following nsPEF treatment.
Myocardial cells were treated with a self-made solid-state LTD high-voltage nanosecond pulse generator with a pulse width of 100 ns for 80 times. The changes in mitochondrial membrane potential and cell apoptosis in rat myocardial cells after nsPEFs were investigated using JC-1 assay kit, apoptosis double staining assay kit, and mitochondrial fluorescence probe.
The results showed that after nsPEF treatment, the mitochondrial membrane potential decreased, apoptosis increased, and the average mitochondrial area decreased from 0.48 µm in live myocardial cells to 0.16 µm. The average circumference ranges from 3.17 µm dropped to 1.60 µm. The shape factor decreased from 1.92 to 1.41. The aspect ratio has decreased from 2.16 to 1.59. nsPEF treatment induces changes in the morphology of myocardial cell mitochondria.
Based on the results of mitochondrial membrane potential and apoptosis, it can be inferred that under this equipment and parameter conditions, nsPEF treatment first causes changes in mitochondrial morphology, and then initiates the mitochondrial apoptosis pathway, which may provide experimental basis for investigating the potential mechanism of nsPEF ablation of myocardial cells.
作为一种新兴的心肌消融技术，纳秒级脉冲电场（nsPEF）消融的机制目前研究较少。线粒体是细胞中重要的膜结构细胞器之一，参与细胞内众多生命活动。本研究旨在探讨纳秒级脉冲电场处理后活细胞中线粒体的形态变化。
用自制的脉冲宽度为100 ns的固态LTD高压纳秒脉冲发生器对心肌细胞进行80次处理。使用JC-1检测试剂盒、凋亡双染检测试剂盒和线粒体荧光探针研究纳秒级脉冲电场处理后大鼠心肌细胞线粒体膜电位和细胞凋亡的变化。
结果表明，纳秒级脉冲电场处理后，线粒体膜电位降低，凋亡增加，活心肌细胞中线粒体平均面积从0.48 µm降至0.16 µm。平均周长从3.17 µm降至1.60 µm。形状因子从1.92降至1.41。纵横比从2.16降至1.59。纳秒级脉冲电场处理诱导心肌细胞线粒体形态发生变化。
基于线粒体膜电位和凋亡结果，可以推断在该设备和参数条件下，纳秒级脉冲电场处理首先引起线粒体形态变化，然后启动线粒体凋亡途径，这可能为研究纳秒级脉冲电场消融心肌细胞的潜在机制提供实验依据。
