Yin Shengyong, Miao Xudong, Zhang Xueming, Chen Xinhua, Wen Hao
Key Laboratory of Combined Multi-Organ Transplantation, The Department of Hepatobiliary and Pancreatic Surgery, Ministry of Public Health and Key Laboratory of Organ Transplantation of Zhejiang Province, The First Affiliated Hospital, Zhejiang University, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
The Department of Orthopedics, the Second Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, China.
J Cell Physiol. 2018 Feb;233(2):1179-1190. doi: 10.1002/jcp.25984. Epub 2017 Jun 6.
Nanosecond pulsed electric field (nsPEF) is a novel non-thermal tumor ablation technique. However, how nsPEF affect cell physiology at different environmental temperature is still kept unknown. But this issue is of critical clinical practice relevance. This work aim to investigate how nsPEF treated cancer cells react to different environmental temperatures (0, 4, 25, and 37°C). Their cell viability, apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) were examined. Lower temperature resulted in higher apoptosis rate, decreased mitochondria membrane potential, and increased ROS levels. Sucrose and N-acetylcysteine (NAC) pre-incubation inhibit ROS generation and increase cell survival, protecting nsPEF-treated cells from low temperature-caused cell death. This work provides an experimental basis for hypothermia and fluid transfusion during nsPEF ablation with anesthesia.
纳秒级脉冲电场（nsPEF）是一种新型的非热肿瘤消融技术。然而，nsPEF在不同环境温度下如何影响细胞生理学仍不清楚。但这个问题具有至关重要的临床实践意义。这项工作旨在研究经nsPEF处理的癌细胞对不同环境温度（0、4、25和37°C）的反应。检测了它们的细胞活力、凋亡、线粒体膜电位和活性氧（ROS）。较低温度导致较高的凋亡率、线粒体膜电位降低和ROS水平升高。蔗糖和N-乙酰半胱氨酸（NAC）预孵育可抑制ROS生成并提高细胞存活率，保护经nsPEF处理的细胞免受低温导致的细胞死亡。这项工作为nsPEF消融联合麻醉期间的低温和液体输注提供了实验依据。
