Zhang Ruiqing, Aji Tuerganaili, Shao Yingmei, Jiang Tiemin, Yang Lei, Lv Weimin, Chen Yonggang, Chen Xinhua, Wen Hao
Hepatobiliary and Hydatid Department, Digestive and Vascular Surgery Centre, Xinjiang Key Laboratory of Echinococcosis, The First Affiliated Hospital of Xinjiang Medical University, #137 Liyushan Road, Urumqi, Xinjiang, 830054, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, #79 Qingchun Road, Zhejiang, Hangzhou, 310003, China.
Parasitol Res. 2017 Apr;116(4):1345-1351. doi: 10.1007/s00436-017-5412-3. Epub 2017 Feb 24.
The number of interventional treatments for hepatic cystic echinococcosis is increasing, but the chemicals or high temperatures used in these methodologies cause biliary complications, thus limiting their clinical applications. This experimental study aimed to apply a novel, non-thermal, non-chemical ablation method termed nanosecond pulsed electric field (nsPEF) for the treatment of human hepatic cystic echinococcosis. The nsPEF treatment parameters against protoscolices from human hepatic cystic echinococcosis were optimized in vitro. The efficacy and mechanism of nsPEF treatment were also investigated. Fresh protoscolices were isolated from human hepatic cystic echinococcosis and were exposed to 300 ns of nsPEF with different field strengths (0, 7, 14, 21, and 29 kV/cm) and pulse numbers (50 and 100 pulses). Then, the viability of the nsPEF-treated protoscolices was evaluated in vitro. Morphological and ultra-structural changes were visualized with H&E staining and scanning electron microscopy. The membrane enzyme activity of alkaline phosphatase (AP) and gamma-glutamyl-transpeptidase (GGT) was measured. nsPEF caused dose-dependent protoscolex death. One-hundred pulses of nsPEF at 21 kV/cm or higher caused a significant increase in the death rate of protoscolices. nsPEF induced significant lethal damage with 50 pulses at 21 or 29 kV/cm and with 100 pulses at 14, 21, or 29 kV/cm, accompanied by morphological destruction and increased levels of AP and GGT membrane enzymes. Thus, nsPEF induced dose-dependent protoscolex mortality and caused destruction of protoscolices and increased membrane enzymes. The mechanism may involve direct damage to the membrane structures of the protoscolices, promoting enzyme exhaustion and disruption of metabolism.
肝囊性棘球蚴病的介入治疗数量正在增加，但这些方法中使用的化学物质或高温会导致胆道并发症，从而限制了它们的临床应用。本实验研究旨在应用一种新型的、非热、非化学的消融方法——纳秒脉冲电场（nsPEF）来治疗人类肝囊性棘球蚴病。在体外优化了针对人类肝囊性棘球蚴病原头节的nsPEF治疗参数。还研究了nsPEF治疗的疗效和机制。从人类肝囊性棘球蚴病中分离出新鲜的原头节，并将其暴露于不同场强（0、7、14、21和29 kV/cm）和脉冲数（50和100个脉冲）的300 ns nsPEF中。然后，在体外评估经nsPEF处理的原头节的活力。通过苏木精-伊红染色和扫描电子显微镜观察形态和超微结构变化。测量碱性磷酸酶（AP）和γ-谷氨酰转肽酶（GGT）的膜酶活性。nsPEF导致原头节死亡呈剂量依赖性。在21 kV/cm或更高场强下施加100个脉冲的nsPEF会导致原头节死亡率显著增加。在21或29 kV/cm下施加50个脉冲以及在14、21或29 kV/cm下施加100个脉冲的nsPEF会引起显著的致死性损伤，同时伴有形态破坏以及AP和GGT膜酶水平升高。因此，nsPEF诱导原头节死亡率呈剂量依赖性，并导致原头节破坏和膜酶增加。其机制可能涉及对原头节膜结构的直接损伤，促进酶耗竭和代谢紊乱。
