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建立脱髓鞘动物模型以研究成年啮齿动物海马神经发生和认知功能的方案。

Protocol to establish a demyelinated animal model to study hippocampal neurogenesis and cognitive function in adult rodents.

机构信息

Department of Neurology, The Third Affiliated Hospital of SUN Yat-sen University, 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China.

Department of Neurology, The Third Affiliated Hospital of SUN Yat-sen University, 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China.

出版信息

STAR Protoc. 2024 Sep 20;5(3):103242. doi: 10.1016/j.xpro.2024.103242. Epub 2024 Aug 1.

DOI:10.1016/j.xpro.2024.103242
PMID:39093706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11342265/
Abstract

Cognitive dysfunction is a prevalent feature in multiple sclerosis, a chronic inflammatory demyelinating disease, which may be correlated with the impairment of adult hippocampal neurogenesis. Here, we present a detailed protocol for the induction of cuprizone demyelinated mice to assess the cognitive function and explore the precise mechanisms underlying cognitive deficits in demyelinated hippocampus. We describe steps for behavioral tests, 5-Ethynyl-2'-deoxyuridine (EdU) and bromodeoxyuridine (BrdU) administration, retrovirus packaging and stereotactic injection, hippocampal tissue preparation, and immunofluorescence staining. For complete details on the use and execution of this protocol, please refer to Song et al..

摘要

认知功能障碍是多发性硬化症（一种慢性炎症性脱髓鞘疾病）的常见特征，其可能与成年海马神经发生受损有关。在此，我们提供了诱导氯化铜致脱髓鞘小鼠的详细方案，以评估其认知功能，并探讨脱髓鞘海马认知缺陷的具体机制。我们描述了行为测试、5-乙炔基-2'-脱氧尿苷（EdU）和溴脱氧尿苷（BrdU）给药、逆转录病毒包装和立体定位注射、海马组织准备和免疫荧光染色的步骤。如需详细了解本方案的使用和实施，请参考 Song 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/53ca323577da/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/d125f4a5f3ac/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/d4c12b3bb55a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/bb149d83cdb6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/a8afee6dda14/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/53ca323577da/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/d125f4a5f3ac/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/d4c12b3bb55a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/bb149d83cdb6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/a8afee6dda14/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07fb/11342265/53ca323577da/gr4.jpg

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