Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
Ultrason Sonochem. 2024 Oct;109:107004. doi: 10.1016/j.ultsonch.2024.107004. Epub 2024 Jul 29.
In this study, the study on physicochemical, rheological properties and water-holding capacity of gelatin of chicken lungs was investigated to replace bovine and porcine gelatin. The extraction rates of chicken, bovine and porcine lung gelatin by ultrasound assisted alkaline protease were 52.12 %, 69.06 % and 70 %, respectively. Three lung gelatins had similar molecular weight distribution in SDS-PAGE with low content of high molecular weight subunits. The amino acid content of bovine lung gelatin (18.03 %) was higher than in chicken (16.62 %) and porcine lung (15.30 %). The highest intensity of 2θ = 7.5° diffraction peak in bovine lung gelatin was observed, which indicated that the triple helix content of bovine lung gelatin was higher than that of chicken and porcine lung gelatin. The lowest apparent viscosity of chicken lung gelatin was 0.253 mPa·s, but the highest water holding capacity of chicken lung gelatin was 331.72 %. Therefore, chicken lung gelatin can be used as a substitute for bovine and porcine gelatin in some functional properties.
本研究旨在探讨鸡肺明胶的理化性质、流变学特性和持水能力，以替代牛和猪明胶。超声辅助碱性蛋白酶提取鸡、牛和猪肺明胶的提取率分别为 52.12%、69.06%和 70%。SDS-PAGE 中三种肺明胶的分子量分布相似，高分子量亚基含量较低。牛肺明胶（18.03%）的氨基酸含量高于鸡（16.62%）和猪（15.30%）。牛肺明胶的 2θ=7.5°衍射峰强度最高，表明牛肺明胶的三螺旋含量高于鸡和猪肺明胶。鸡肺明胶的表观粘度最低，为 0.253 mPa·s，但鸡肺明胶的持水能力最高，为 331.72%。因此，鸡肺明胶可以在某些功能特性上替代牛和猪明胶。
