从斑点叉尾鮰（Ictalurus punctatus）皮中提取胶原蛋白的分离与特性分析。

Isolation and characterization of collagen extracted from channel catfish (Ictalurus punctatus) skin.

机构信息

Department of Food Science, Nutrition and Health Promotion, Mississippi State University, Mississippi State, MS 39762, United States.

出版信息

Food Chem. 2018 Mar 1;242:147-155. doi: 10.1016/j.foodchem.2017.09.013. Epub 2017 Sep 7.

DOI:10.1016/j.foodchem.2017.09.013

PMID:29037670

Abstract

Channel catfish skin is a by-product from catfish fillet production. Collagens were extracted from catfish skins by: (1) acid; (2) homogenization-aided; and (3) pepsin-aided extraction methods. Kinetic analysis of extraction was performed. SDS-PAGE was carried out for all collagens extracted under different conditions. Protein solubility, zeta potential, circular dichroism and gel strength of the collagen extracted by three methods were studied to determine optimal extraction conditions. Protein recovery rate from minced skins extracted with pH 2.4 HCl containing 23.6KU/g pepsin was the highest (64.19%). SDS-PAGE showed that collagens extracted with different methods had different proteins ratio patterns, even though the molecular mass of collagen subunits were similar, 123 and 113KDa for α and α chains, 226KDa for β chain and 338.5KDa for γ chain, respectively. Channel catfish skin collagens were typical type I collagens and could have applications in food, medical and cosmetic industries.

摘要

鲶鱼皮是从鲶鱼片生产过程中的副产品。胶原蛋白是通过以下三种方法从鲶鱼皮中提取的：（1）酸法；（2）匀浆辅助法；（3）胃蛋白酶辅助提取法。对提取的动力学进行了分析。对不同条件下提取的所有胶原蛋白进行 SDS-PAGE 分析。研究了三种方法提取的胶原蛋白的蛋白质溶解度、ζ 电位、圆二色性和凝胶强度，以确定最佳提取条件。用 pH 2.4 HCl 提取，其中含有 23.6KU/g 胃蛋白酶的切碎鱼皮的蛋白质回收率最高（64.19%）。SDS-PAGE 显示，用不同方法提取的胶原蛋白具有不同的蛋白质比例模式，尽管胶原蛋白亚基的分子量相似，α和α链为 123 和 113 kDa，β链为 226 kDa，γ链为 338.5 kDa。鲶鱼皮胶原蛋白是典型的 I 型胶原蛋白，可应用于食品、医疗和化妆品行业。