Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, No. 44-1 Wenhua Road West, Jinan, Shandong 250012, China.
Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, No. 44-1 Wenhua Road West, Jinan, Shandong 250012, China.
Arch Oral Biol. 2024 Nov;167:106062. doi: 10.1016/j.archoralbio.2024.106062. Epub 2024 Jul 30.
Alveolar bone quality is essential for the maxillofacial integrity and function, and depends on alveolar bone mineralization. This study aims to investigate the in vivo changes in alveolar bone mineralization, from the perspective of mineral deposition and crystal transition in postnatal rats.
Nine postnatal time points of Wistar rats, ranging from day 1 to 56, were set to obtain the maxillary alveolar bone samples. Each time point consisted of ninety rats, with 45 females and 45 males. Macromorphology of alveolar bone was reconducted by Micro-Computed Tomography and the mineral content was quantified via Thermogravimetric analysis, Scanning Electron Microscope, High-Resolution Transmission Electron Microscopy and vibrational spectroscopy. Furthermore, the crystallinity and composition were characterized by vibrational spectroscopy, X-ray Diffraction, X-ray Photoelectron Spectroscopy and Selected Area Electron Diffraction.
The progressive increase of mineral deposition was accompanied by substantial growth in alveolar bone mass and volume in postnatal rats. Whereas the mineral percentage initially decreased and then increased, reaching a nadir on postnatal day 14 (P14) when tooth eruption was first observed. Besides, localized mineralization was initiated by the formation of amorphous precursors and then converted into mineral crystals, while there was no statistically significant change in the average crystallinity of the bone during growth.
Mineralization of alveolar bone is ongoing throughout the early growth in postnatal rats. Mineral deposition increases with age, whereas the crystallinity remains stable within a certain range. Besides, the mineral percentage reaches its lowest point on P14, which may be attributed to tooth eruption.
牙槽骨质量对颌面部的完整性和功能至关重要，而牙槽骨质量取决于牙槽骨矿化。本研究旨在从新生大鼠矿化过程中矿物质沉积和晶体转变的角度，研究牙槽骨矿化的体内变化。
设置了 9 个新生大鼠时间点，范围从第 1 天到第 56 天，以获得上颌牙槽骨样本。每个时间点包括 90 只大鼠，其中 45 只为雌性，45 只为雄性。采用 Micro-Computed Tomography 进行牙槽骨的宏观形态重建，并通过热重分析、扫描电子显微镜、高分辨率透射电子显微镜和振动光谱法定量分析矿物质含量。此外，采用振动光谱法、X 射线衍射、X 射线光电子能谱和选区电子衍射法对结晶度和成分进行了表征。
在新生大鼠中，矿物质沉积的逐渐增加伴随着牙槽骨质量和体积的显著增长。尽管矿物质百分比最初降低然后增加，但在观察到牙齿萌出的第 14 天（P14）时达到最低点。此外，局部矿化是由无定形前体的形成开始，然后转化为矿物质晶体，而在生长过程中，骨的平均结晶度没有统计学上的显著变化。
牙槽骨矿化在新生大鼠的早期生长过程中持续进行。矿物质沉积随年龄增长而增加，而结晶度在一定范围内保持稳定。此外，矿物质百分比在 P14 时达到最低点，这可能与牙齿萌出有关。
