Mesoporous silica thin film as effective coating for enhancing osteogenesis through selective protein adsorption and blood clotting.

The role of blood clots in tissue repair has been identified for a long time; however, its participation in the integration between implants and host tissues has attracted attention only in recent years. In this work, a mesoporous silica thin film (MSTF) with either vertical or parallel orientation was deposited on titania nanotubes surface, resulting in superhydrophilic nanoporous surfaces. A proteomic analysis of blood plasma adsorption revealed that the MSTF coating could significantly increase the abundance of acidic proteins and the adsorption of coagulation factors (XII and XI), with the help of cations (Na, Ca) binding. As a result, both the activation of platelets and the formation of blood clots were significantly enhanced on the MSTF surface with more condensed fibrin networks. The two classical growth factors of platelets-derived growth factors-AB and transformed growth factors-were enriched in blood clots from the MSTF surface, which accounted for robust osteogenesis bothand. This study demonstrates that MSTF may be a promising coating to enhance osteogenesis by modulating blood clot formation.