Department of Biomaterials Science, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8544, Japan.
Department of Restorative Dentistry and Endodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8544, Japan.
Biomed Mater. 2024 Aug 14;19(5). doi: 10.1088/1748-605X/ad6ac3.
Because of its superior strength, esthetic properties, and excellent biocompatibility, zirconia is preferred for dental prosthetic such as crowns and bridges. However, zirconia crowns and bridges are susceptible to secondary caries owing to margin leakage. Silver is a well-known antibacterial agent, making it a desirable additive to zirconia crowns and bridges for secondary caries prevention. This study focuses on imparting zirconia composite with antibacterial properties to enhance its protective capacity in dental restorations. We used the sol-gel method to dope Ag into zirconia. Silver-doped zirconia powders were prepared at Zr:Ag molar ratios of 100:0,100:0.1, 100:0.5, 100:1, 100:3, and 100:5 (respective samples denoted as Ag-0, Ag-0.1, Ag-0.5, Ag-1, Ag-3, and Ag-5) and were subjected to firing at various temperatures (400 °C-1000 °C). We performed x-ray diffraction to investigate the crystal phase of these powders and x-ray fluorescence and field emission scanning electron microscopy to analyze their elemental composition and surface morphology, respectively. Moreover, we performed spectrophotometry to determine the*** color values, conducted dissolution tests, and quantified the Ag content through inductively coupled plasma optical emission spectroscopy. In addition, we studied the antibacterial activity of the samples. Analyses of the samples fired at ⩽600 °C revealed a predominantly white to grayish-white coloration and a tetragonal crystal phase. Firing at ⩾700 °C resulted in gray or dark gray coloration and a monoclinic crystal phase. The Ag content decreased after firing at 900 °C or 1000 °C. Ag-0.5 and above exhibited antibacterial activity against bothand. Therefore, the minimum effective silver-doped zirconia sample was found to be Ag-0.5. This study allows the exploration of the antimicrobial potential of silver-doped zirconia materials in dental applications such as prosthdontical lining materials, promoting the development of innovative restorations with protective capacity against secondary caries.
由于其优异的强度、美观性能和出色的生物相容性，氧化锆被优先用于牙科修复体，如牙冠和牙桥。然而，氧化锆牙冠和牙桥容易发生边缘渗漏，导致继发龋。银是一种众所周知的抗菌剂，因此将其添加到氧化锆牙冠和牙桥中以预防继发龋是一种理想的方法。本研究旨在赋予氧化锆复合材料抗菌性能，以提高其在牙科修复中的保护能力。我们使用溶胶-凝胶法将银掺杂到氧化锆中。制备了氧化锆：银摩尔比为 100:0、100:0.1、100:0.5、100:1、100:3 和 100:5（分别表示为 Ag-0、Ag-0.1、Ag-0.5、Ag-1、Ag-3 和 Ag-5）的银掺杂氧化锆粉末，并在不同温度（400°C-1000°C）下进行煅烧。我们通过 X 射线衍射研究了这些粉末的晶体相，通过 X 射线荧光和场发射扫描电子显微镜分别分析了它们的元素组成和表面形貌。此外，我们还进行了分光光度法测定颜色值、溶解试验和电感耦合等离子体发射光谱法定量分析银含量。此外，我们研究了样品的抗菌活性。在 ⩽600°C 下烧制的样品呈现出白色到灰白色为主的颜色和四方晶相。在 ⩾700°C 下烧制的样品呈现出灰色或深灰色的颜色和单斜晶相。在 900°C 或 1000°C 烧制后，银含量下降。Ag-0.5 及以上对均表现出抗菌活性。因此，最小有效银掺杂氧化锆样品为 Ag-0.5。本研究为探索银掺杂氧化锆材料在牙科应用中的抗菌潜力提供了依据，例如作为修复体衬里材料，促进具有抗继发龋能力的创新修复体的发展。
