Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani, Thailand.
Behav Brain Res. 2024 Oct 2;474:115172. doi: 10.1016/j.bbr.2024.115172. Epub 2024 Jul 31.
The phytoestrogens daidzein and genistein are ubiquitous in human food. This study aimed to elucidate their anxiety-liked effects, their effects on the reproductive organs, and the molecular mechanism behind any anxiety-liked effects in intact adult male Wistar rats. These phytoestrogens are of interest due to their posited health benefits, particularly for female, but with some effect on males as well. This study comprised two experiments: (1) Male Wistar rats received either a vehicle, daidzein, or genistein (0.25, 0.50, or 1.00 mg/kg) by subcutaneously injection for four weeks. They were then tested for anxiety-liked behaviors. Then, the brain monoamines in anxiolytic rats were determined; (2) The modulation of gamma aminobutyric acid receptors by phytoestrogens was further analyzed by administration of diazepam to phytoestrogen-treated rats before behavioral tests. In the first experiment, the biological parameters measured, including body weight, daily food intake and reproductive organ weights were unaffected by either genistein or daidzein. However, anxiolytic-like effect was observed in the low-dose daidzein (0.25 mg/kg) group. Higher doses of daidzein or genistein of all doses had no effect. Further, the low-dose daidzein did not alter brain monoamine levels. In the second experiment, the anxiolytic-like behavior of daidzein-treated rats receiving diazepam did not differ from that of the rats treated with just diazepam or just daidzein. In conclusion, 4-week exposure to daidzein or genistein had no negative effects on the reproductive organs, body weight, food intake, anxiogenic-like behavior, or monoaminergic and diazepam-modulated GABAergic neurotransmissions of intact male rats. However, beneficial anxiolytic-like effects were apparent after low-dose treatment with daidzein.
植物雌激素大豆苷元和染料木黄酮普遍存在于人类食物中。本研究旨在阐明它们的类似焦虑作用、对生殖器官的影响，以及任何类似焦虑作用的分子机制在完整成年雄性 Wistar 大鼠中。由于它们假定的健康益处，特别是对女性，但对男性也有一些影响，这些植物雌激素引起了人们的兴趣。本研究包括两个实验：（1）雄性 Wistar 大鼠接受皮下注射溶剂、大豆苷元或染料木黄酮（0.25、0.50 或 1.00mg/kg），共四周。然后对其进行类似焦虑行为测试。然后，在抗焦虑大鼠中测定脑单胺；（2）通过给予植物雌激素处理的大鼠地西泮，进一步分析植物雌激素对γ-氨基丁酸受体的调制，然后进行行为测试。在第一个实验中，测量的生物学参数，包括体重、每日食物摄入量和生殖器官重量，不受大豆苷元或染料木黄酮的影响。然而，在低剂量大豆苷元（0.25mg/kg）组中观察到类似焦虑的作用。所有剂量的高剂量大豆苷元和染料木黄酮均无作用。此外，低剂量大豆苷元并未改变脑单胺水平。在第二个实验中，接受地西泮的大豆苷元处理大鼠的类似焦虑行为与仅接受地西泮或仅接受大豆苷元的大鼠没有区别。总之，4 周暴露于大豆苷元或染料木黄酮对雄性大鼠的生殖器官、体重、食物摄入、焦虑样行为、单胺能和地西泮调节的 GABA 能神经传递无负面影响。然而，低剂量大豆苷元处理后出现了有益的类似抗焦虑作用。
