Laboratorio de Neurofarmacología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, VER, Mexico.
Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, Xalapa, VER, Mexico.
Biomed Res Int. 2017;2017:9073816. doi: 10.1155/2017/9073816. Epub 2017 Oct 31.
The phytoestrogen genistein produces anxiolytic-like effects in ovariectomized rats, which highlights its potential therapeutic effect in ameliorating anxiety in surgical menopausal women. However, no studies have directly compared the effects of identical doses of genistein and 17-estradiol, the main estrogen used in hormone replacement therapy in menopausal women. The present study evaluated the anxiolytic-like effects of identical doses of genistein and 17-estradiol (0.045, 0.09, and 0.18 mg/kg/7 days, s.c.) in a surgical menopause model in rats in the elevated plus maze and locomotor activity tests at 12 weeks after ovariectomy. Additionally, the participation of estrogen receptor- in the anxiolytic-like effect of genistein and 17-estradiol was explored by previous administration of the 5 mg/kg tamoxifen antagonist. Genistein and 17-estradiol (0.09 and 0.18 mg/kg) similarly reduced anxiety-like behavior in the elevated plus maze and also increased the time spent grooming and rearing, without affecting crossing in locomotor activity test. These effects were blocked by tamoxifen. Present results indicate that the phytoestrogen genistein has a similar behavioral profile as 17-estradiol in rats at 12 weeks after ovariectomy through action at the estrogen receptor-. Thus genistein has potential for reducing anxiety-like behavior associated with low concentrations of ovarian hormones, which normally occurs during natural and surgical menopause.
植物雌激素金雀异黄素可产生类似抗焦虑的作用，这突出了其在改善手术绝经后女性焦虑症方面的潜在治疗作用。然而，目前还没有研究直接比较相同剂量的金雀异黄素和 17-雌二醇（17-estradiol）的作用，17-雌二醇是绝经后激素替代疗法中使用的主要雌激素。本研究在卵巢切除后 12 周，通过高架十字迷宫和运动活性测试，评估了相同剂量的金雀异黄素（0.045、0.09 和 0.18mg/kg/7 天，皮下注射）和 17-雌二醇（0.09 和 0.18mg/kg）对手术性绝经模型大鼠的类似抗焦虑作用。此外，通过预先给予 5mg/kg 他莫昔芬拮抗剂，探索了雌激素受体在金雀异黄素和 17-雌二醇的类似抗焦虑作用中的参与。金雀异黄素和 17-雌二醇（0.09 和 0.18mg/kg）同样减少了高架十字迷宫中的焦虑样行为，同时增加了梳理和站立的时间，而对运动活性测试中的穿越行为没有影响。这些作用被他莫昔芬阻断。目前的结果表明，金雀异黄素在卵巢切除后 12 周的大鼠中具有与 17-雌二醇相似的行为特征，通过雌激素受体作用。因此，金雀异黄素具有减少与卵巢激素浓度降低相关的焦虑样行为的潜力，这种情况通常发生在自然和手术绝经期间。
