Chen Lin, Hu Menglu, Zhou Xiaoming
School of Life sciences, South China Normal University, Guangzhou 510631, P. R. China.
School of Life sciences, South China Normal University, Guangzhou 510631, P. R. China.
Trends Biotechnol. 2025 Jan;43(1):98-110. doi: 10.1016/j.tibtech.2024.07.007. Epub 2024 Aug 2.
The integration of nucleic acid amplification (NAA) with the CRISPR detection system has led to significant advancements and opportunities for development in molecular diagnostics. Nevertheless, the incompatibility between CRISPR cleavage and NAA has significantly impeded the commercialization of this technology. Currently, several one-pot detection strategies based on CRISPR systems have been devised to address concerns regarding aerosol contamination risk and operational complexity associated with step-by-step detection as well as the sensitivity limitation of conventional one-pot methods. In this review, we provide a comprehensive introduction and outlook of the various solutions of the one-pot CRISPR assay for practitioners who are committed to developing better CRISPR nucleic acid detection technologies to promote the progress of molecular diagnostics.
核酸扩增（NAA）与CRISPR检测系统的整合为分子诊断带来了重大进展和发展机遇。然而，CRISPR切割与NAA之间的不兼容性严重阻碍了该技术的商业化。目前，已经设计了几种基于CRISPR系统的一锅法检测策略，以解决与逐步检测相关的气溶胶污染风险和操作复杂性问题，以及传统一锅法的灵敏度限制。在本综述中，我们为致力于开发更好的CRISPR核酸检测技术以推动分子诊断进展的从业者提供了一锅法CRISPR检测各种解决方案的全面介绍和展望。
