Department of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda, Uttar Pradesh, 210 001, India.
Department of Entomology, Banda University of Agriculture and Technology, Banda, Uttar Pradesh, 210 001, India.
Sci Rep. 2024 Aug 2;14(1):17907. doi: 10.1038/s41598-024-68943-1.
Linseed, also known as flax is an important oilseed crop with many potential uses in paint, textile, food and pharmaceutical industries. Susceptibility to bud fly (Dasyneura lini Barnes) infestation is a serious biotic concern leading to severe yield penalty in linseed. Protease inhibitors (PIs) are potential candidates that activate during the insect-pest attack and modulate the resistance. In the present study, we explored the PI candidates in the linseed genome and a total of 100 LuPI genes were identified and grouped into five distinct subgroups. The analysis of cis-acting elements revealed that almost all LuPI promoters contain several regulatory elementary related to growth and development, hormonal regulation and stress responses. Across the subfamilies of PIs, the specific domains are consistently found conserved in all protein sequences. The tissue-specific in-silico expression pattern via RNA-seq revealed that all the genes were regulated during different stress. The expression through qRT-PCR of 15 genes revealed the significant up-regulation of LuPI-24, LuPI-40, LuPI-49, LuPI-53, and LuPI-63 upon bud fly infestation in resistant genotype EC0099001 and resistant check variety Neela. This study establishes a foundation resource for comprehending the structural, functional, and evolutionary dimensions of protease inhibitors in linseed.
亚麻，也被称为亚麻，是一种重要的油料作物，在油漆、纺织、食品和制药等行业有许多潜在的用途。对芽蝇（Dasyneura lini Barnes）感染的敏感性是一个严重的生物问题，导致亚麻产量严重下降。蛋白酶抑制剂（PIs）是潜在的候选物质，它们在昆虫-害虫攻击时被激活，并调节抗性。在本研究中，我们探索了亚麻基因组中的 PI 候选物，共鉴定出 100 个 LuPI 基因，并将其分为五个不同的亚组。顺式作用元件的分析表明，几乎所有 LuPI 启动子都包含几个与生长发育、激素调节和应激反应相关的调节元件。在 PI 的亚科中，所有蛋白序列都一致地发现具有特定的保守结构域。通过 RNA-seq 进行的组织特异性计算机表达模式显示，所有基因在不同的应激条件下都受到调控。通过 qRT-PCR 对 15 个基因的表达显示，在抗性基因型 EC0099001 和抗性对照品种 Neela 中，芽蝇感染后 LuPI-24、LuPI-40、LuPI-49、LuPI-53 和 LuPI-63 的表达显著上调。本研究为理解亚麻蛋白酶抑制剂的结构、功能和进化维度奠定了基础资源。
