Grupo de Investigación en Procesos Agroindustriales (GIPA), Doctorado en Biociencias, Facultad de Ingeniería, Universidad de La Sabana, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chia, Cundinamarca, Colombia.
Sci Rep. 2024 Aug 2;14(1):17955. doi: 10.1038/s41598-024-68783-z.
Probiotic production in commercial culture media is expensive, so, it is necessary to design culture media based on "low-cost" components like agro-industrial by-products. Therefore, this study aimed to design an agro-industrial by-product-based culture media using whey, sugarcane molasses, and palm kernel cake as components to produce Lactococcus lactis A12, Priestia megaterium M4, and Priestia sp. M10 isolated from Nile tilapia (Oreochromis niloticus) associated gut microbiota. Higher bacterial concentrations were achieved at high whey concentrations and low concentrations of sugarcane molasses and palm kernel cake (PKC) using agitation. The optimal conditions were whey, 3.84% w/v; sugarcane molasses, 7.39% w/v; PKC, 0.77% w/v; and agitation speed, 75 RPM. Bacterial growth under optimal conditions was compared to that in commercial Brain-Heart Infusion (BHI) broth. L. lactis A12 showed similar growth in the optimal media and BHI. The estimated cost of the culture media based on component prices was USD $ 3.01/L, which is 86.93% lower than BHI broth (USD $ 23.04/L). It was possible to design a "low-cost agro-industrial by-product-based culture media to produce L. lactis A12 and the two Priestia species under monoculture conditions.
商业培养基于昂贵的成分，所以有必要设计基于“低成本”成分的培养基，如农业工业副产物。因此，本研究旨在设计一种基于乳清、甘蔗废糖蜜和棕仁饼作为成分的农业工业副产物培养基，以生产从尼罗罗非鱼（Oreochromis niloticus）相关肠道微生物群中分离出的乳球菌（Lactococcus lactis）A12、巨拟叶螱（Priestia megaterium）M4 和 Priestia sp. M10。在搅拌下，高乳清浓度和低甘蔗废糖蜜和棕仁饼（PKC）浓度下可获得更高的细菌浓度。最佳条件为乳清，3.84% w/v；甘蔗废糖蜜，7.39% w/v；PKC，0.77% w/v；搅拌速度，75 RPM。在最佳条件下的细菌生长与在商业脑心浸液（BHI）肉汤中的生长进行了比较。L. lactis A12 在最佳培养基和 BHI 中的生长情况相似。基于成分价格的培养基估计成本为 3.01 美元/L，比 BHI 肉汤（23.04 美元/L）低 86.93%。在单一培养条件下，有可能设计一种“低成本农业工业副产物为基础的培养基来生产 L. lactis A12 和两种 Priestia 物种。
