补料分批培养法在富含营养物质的乳清培养基中生产益生菌生物量和乳链菌肽。
Batch and fed-batch production of probiotic biomass and nisin in nutrient-supplemented whey media.
机构信息
Department of Analytical and Food Chemistry, Science Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004, Ourense, Spain.
Department of Engineering, Materials, Structural Mechanics and Construction, Science Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004, Ourense, Spain.
出版信息
Braz J Microbiol. 2019 Oct;50(4):915-925. doi: 10.1007/s42770-019-00114-1. Epub 2019 Aug 5.
The production of a highly concentrated probiotic preparation of Lactococcus lactis CECT 539 was studied in both batch and realkalized fed-batch fermentations in diluted whey (DW) media (DW25, DW50, DW75, DW100, and DW125) supplemented with MRS broth nutrients (except glucose and Tween 80) at 25, 50, 75, 100, and 125% of their standard concentrations in the complex medium. The fed-batch culture using DW100 medium provided the highest concentrations of probiotic biomass (5.98 g/L) and nisin (258.47 BU/mL), which were obtained at lower production costs than those estimated for the fed-batch culture in DW medium. The batch and fed-batch cultures reduced the initial chemical oxygen demand (COD) of the media by 29.1-41.7% and 31.2-54.2%, respectively. Graphical abstract.
研究了在 25%、50%、75%、100%和 125%的标准浓度下，用浓缩乳清（DW）培养基（DW25、DW50、DW75、DW100 和 DW125）代替复杂培养基中的 MRS 肉汤营养成分（除葡萄糖和吐温 80 外），并进行间歇和再碱化分批发酵，生产乳球菌 CECT539 的高浓度益生菌制剂。在 DW100 培养基中进行的分批和补料分批培养，提供了最高的益生菌生物量（5.98 g/L）和乳链菌肽（258.47 BU/mL）浓度，其生产成本低于在 DW 培养基中进行补料分批培养的估计成本。批式和补料分批培养分别将培养基的初始化学需氧量（COD）降低了 29.1-41.7%和 31.2-54.2%。
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