Department of Analytical and Food Chemistry, Science Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004, Ourense, Spain.
Department of Engineering, Materials, Structural Mechanics and Construction, Science Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004, Ourense, Spain.
Braz J Microbiol. 2019 Oct;50(4):915-925. doi: 10.1007/s42770-019-00114-1. Epub 2019 Aug 5.
The production of a highly concentrated probiotic preparation of Lactococcus lactis CECT 539 was studied in both batch and realkalized fed-batch fermentations in diluted whey (DW) media (DW25, DW50, DW75, DW100, and DW125) supplemented with MRS broth nutrients (except glucose and Tween 80) at 25, 50, 75, 100, and 125% of their standard concentrations in the complex medium. The fed-batch culture using DW100 medium provided the highest concentrations of probiotic biomass (5.98 g/L) and nisin (258.47 BU/mL), which were obtained at lower production costs than those estimated for the fed-batch culture in DW medium. The batch and fed-batch cultures reduced the initial chemical oxygen demand (COD) of the media by 29.1-41.7% and 31.2-54.2%, respectively. Graphical abstract.
研究了在 25%、50%、75%、100%和 125%的标准浓度下，用浓缩乳清（DW）培养基（DW25、DW50、DW75、DW100 和 DW125）代替复杂培养基中的 MRS 肉汤营养成分（除葡萄糖和吐温 80 外），并进行间歇和再碱化分批发酵，生产乳球菌 CECT539 的高浓度益生菌制剂。在 DW100 培养基中进行的分批和补料分批培养，提供了最高的益生菌生物量（5.98 g/L）和乳链菌肽（258.47 BU/mL）浓度，其生产成本低于在 DW 培养基中进行补料分批培养的估计成本。批式和补料分批培养分别将培养基的初始化学需氧量（COD）降低了 29.1-41.7%和 31.2-54.2%。
