Division of Paediatric Endocrinology and Diabetes, Department of Paediatrics, University of Patras School of Medicine, Patras, 26504, Greece.
Department of Nursing, Laboratory of Basic Health Sciences, Faculty of Health Sciences, University of Peloponnese, Panarcadian Hospital Erythrou Stavrou End Administrative Services 2 Floor, Tripoli, 22100, Greece.
BMC Pediatr. 2024 Aug 2;24(1):493. doi: 10.1186/s12887-024-04926-5.
Type 1 diabetes mellitus (T1DM) is characterized by immune and metabolic dysregulation. Apo1/Fas is implicated in maintaining homeostasis of the immune system. Cytokeratin-18 (cCK-18) is a predictive marker of liver disorders in T2DM. Intercellular adhesion molecule-1 (ICAM-1) is considered to increase susceptibility to diabetes mellitus. All three markers are associated with endothelial function, apoptosis and diabetes-related complications. The possible role of Apo1/Fas, cCK-18 and ICAM-1 was investigated in children and adolescents with T1DM.
Forty-nine (49) children and adolescents with T1DM and 49 controls were included in the study. Somatometric measurements were obtained and the Body Mass Index (BMI) of the participants was calculated. Biochemical parameters were measured by standard laboratory methods and Apo1/Fas, cCK-18 and ICAM-1 were measured using appropriate ELISA kits. The statistical analysis was performed using the IBM SPSS Statistics 23 program.
Apo1/Fas (p = 0.001), cCK-18 (p < 0.001) and ICAM-1 (p < 0.001) were higher in patients with T1DM compared to the controls. Apo1Fas was negatively correlated with glucose (p = 0.042), uric acid (p = 0.026), creatinine (p = 0.022), total cholesterol (p = 0.023) and LDL (p = 0.005) in the controls. In children and adolescents with T1DM, Apo1/Fas was positively correlated with total cholesterol (p = 0.013) and LDL (p = 0.003). ICAM-1 was negatively correlated with creatinine (p = 0.019) in the controls, whereas in patients with T1DM it was negatively correlated with HbA1c (p = 0.05).
Apo1/Fas, cCK-18 and ICAM-1 may be useful as serological markers for immune and metabolic dysregulation in children and adolescents with T1DM. Also, Apo1/Fas may have a protective role against metabolic complications in healthy children.
1 型糖尿病（T1DM）的特征是免疫和代谢失调。Apo1/Fas 参与维持免疫系统的稳态。细胞角蛋白-18（cCK-18）是 2 型糖尿病肝损伤的预测标志物。细胞间黏附分子-1（ICAM-1）被认为增加了患糖尿病的易感性。所有这三种标志物都与内皮功能、细胞凋亡和糖尿病相关并发症有关。在儿童和青少年 1 型糖尿病患者中研究了 Apo1/Fas、cCK-18 和 ICAM-1 的可能作用。
纳入 49 例 1 型糖尿病患儿和 49 例对照者。进行体格测量，并计算参与者的体重指数（BMI）。采用标准实验室方法测定生化参数，采用适当的 ELISA 试剂盒测定 Apo1/Fas、cCK-18 和 ICAM-1。采用 IBM SPSS Statistics 23 程序进行统计分析。
与对照组相比，1 型糖尿病患儿的 Apo1/Fas（p=0.001）、cCK-18（p<0.001）和 ICAM-1（p<0.001）水平更高。在对照组中，Apo1Fas 与血糖（p=0.042）、尿酸（p=0.026）、肌酐（p=0.022）、总胆固醇（p=0.023）和 LDL（p=0.005）呈负相关。在 1 型糖尿病患儿中，Apo1/Fas 与总胆固醇（p=0.013）和 LDL（p=0.003）呈正相关。在对照组中，ICAM-1 与肌酐（p=0.019）呈负相关，而在 1 型糖尿病患儿中，ICAM-1 与 HbA1c（p=0.05）呈负相关。
Apo1/Fas、cCK-18 和 ICAM-1 可用作 1 型糖尿病患儿免疫和代谢失调的血清学标志物。此外，Apo1/Fas 可能对健康儿童的代谢并发症具有保护作用。
