Department of Pediatric Dentistry, Faculty of Dentistry, Başkent University, Ankara, Türkiye.
Department of Medical Microbiology, Faculty of Medicine, Başkent University, Ankara, Türkiye.
BMC Oral Health. 2024 Aug 2;24(1):886. doi: 10.1186/s12903-024-04659-y.
Probiotics are live beneficial bacteria to human health and their efficiency on oral health is still being investigated. The purpose of this study was to evaluate the level of Streptococcus mutans and Lactobacillus species with and without the use of probiotics for six-months after the treatment of all dental caries under general anesthesia.
Fifty-eight pediatric patients without any systemic diseases, whose dental treatments were completed under general anesthesia (GA), were included in the study. The patients were recruited in two-groups; Group A: Patients started using probiotics after GA and Group B: Patients did not use probiotics after GA. Saliva samples were taken from all patients on the day before GA (T0), at one-month (T1), three-month (T2) and six-month (T3) follow-up after GA. The counts of cariogenic bacteria were determined by the analysis of saliva samples using real-time polymerase chain reaction. Statistical significance level was accepted as p < 0.05.
There was statistically significant difference between Group A and B for T0, T1, T2 and T3 regarding S. mutans (p = 0.001, p = 0.04, p = 0.04, p = 0.03; p < 0.05). However, there was no statistically significant difference between groups regarding Lactobacillus species (p ≥ 0.05).
Probiotic use and treatment of all caries significantly reduced the level of S. mutans but not Lactobacillus species. Furthermore, S. mutans decreased after cessation of probiotics, but it was not statistically significant.
Study was registered as "Effects of Probiotics on Streptococcus mutans and Lactobacillus species" with the registration number of NCT05859646 (16/05/2023) at https://www.
gov Protocol Registration and Results System.
益生菌对人类健康有益，其对口腔健康的功效仍在研究中。本研究旨在评估使用和不使用益生菌治疗全麻下所有龋齿后 6 个月时变形链球菌和乳酸杆菌的水平。
本研究纳入 58 例无任何系统性疾病的儿科患者，全麻下完成口腔治疗。将患者分为两组；A 组：患者在全麻后开始使用益生菌，B 组：患者在全麻后不使用益生菌。所有患者在全麻前一天（T0）、术后 1 个月（T1）、3 个月（T2）和 6 个月（T3）采集唾液样本。通过实时聚合酶链反应分析唾液样本，确定致龋菌的数量。统计显著性水平为 p < 0.05。
A 组和 B 组在 T0、T1、T2 和 T3 时，S. mutans 的数值有统计学差异（p = 0.001，p = 0.04，p = 0.04，p = 0.03；p < 0.05）。然而，两组之间的乳酸杆菌数值没有统计学差异（p ≥ 0.05）。
益生菌使用和所有龋齿的治疗显著降低了 S. mutans 的水平，但对乳酸杆菌的水平没有影响。此外，停用益生菌后 S. mutans 水平下降，但无统计学意义。
该研究在 https://www.clinicaltrials.gov 以“益生菌对变形链球菌和乳酸杆菌的影响”（注册号 NCT05859646）进行注册。
NCT05859646
