College of Nursing and Health, Henan University, Kaifeng, Henan, China.
Henan Engineering Research Center of Industrial Recirculating Water Treatment, College of Chemistry and Molecular Sciences, Henan University, Kaifeng, Henan, China.
Appl Spectrosc. 2024 Jul;78(7):744-752. doi: 10.1177/00037028241241342. Epub 2024 Apr 1.
Hemicyanine dyes are an ideal structure for building near-infrared fluorescent probes due to their excellent emission wavelength properties and biocompatibility in biological imaging field. Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A novel developed fluorescent probe P showed high selectivity and sensitivity to Cys in the presence of various analytes. The detection limit of P was found to be 0.329 μM. The MTT assay showed that the probe was essentially non-cytotoxic. Furthermore, the probe was successfully used as cysteine imaging in living cells and mice.
花菁染料由于其在生物成像领域具有优异的发射波长性质和生物相容性，是构建近红外荧光探针的理想结构。本研究旨在开发一种能够检测半胱氨酸 (Cys) 的近红外荧光探针。新开发的荧光探针 P 在存在各种分析物的情况下对 Cys 表现出高选择性和高灵敏度。发现 P 的检测限为 0.329 μM。MTT 测定表明该探针基本无细胞毒性。此外，该探针成功地用于活细胞和小鼠中的半胱氨酸成像。
