School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
Tianjin Key Laboratory for Photoelectric Materials and Devices, and Key Laboratory of Display Materials & Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
J Mater Chem B. 2020 Mar 18;8(11):2269-2274. doi: 10.1039/c9tb02609f.
Cysteine (Cys) is one of the most important essential biothiols in lysosomes. Highly selective probes for specific detection and imaging of lysosomal Cys over other biological thiols are rare. Herein, we developed a lysosome-targeted near-infrared fluorescent probe SHCy-C based on a novel NIR-emitting thioxanthene-indolium dye. Due to the turn-on fluorescence response elicited by the intramolecular charge transfer (ICT) processes before and after the reaction with Cys, probe SHCy-C exhibits high selectivity and sensitivity (16 nM) for the detection of Cys. More importantly, probe SHCy-C is found to precisely target lysosomes and achieves the "turn-on" detection and imaging of endogenous Cys in lysosomes.
半胱氨酸（Cys）是溶酶体中最重要的必需生物硫醇之一。用于特异性检测和成像溶酶体 Cys 而不是其他生物硫醇的高选择性探针很少见。在此，我们基于新型近红外发射噻吨-吲哚鎓染料开发了一种溶酶体靶向近红外荧光探针 SHCy-C。由于与 Cys 反应前后分子内电荷转移（ICT）过程引起的荧光响应开启，探针 SHCy-C 对 Cys 的检测具有高选择性和灵敏度（16 nM）。更重要的是，发现探针 SHCy-C 能够精确靶向溶酶体，并实现溶酶体中内源性 Cys 的“开启”检测和成像。
