通过特定的脱氧核酶介导的信号放大构建具有“关-开”模式的上转换荧光传感平台，用于超灵敏检测铀酰离子。
Engineering an upconversion fluorescence sensing platform with "off-on" pattern through specific DNAzyme-mediated signal amplification for supersensitive detection of uranyl ion.
作者信息
Zhang Xinyu, Wang Yue, Gong Mi, Xiong Lihao, Song Jiayi, Chen Sihan, Tong Yuqi, Liu Yu, Li Le, Zhen Deshuai
机构信息
Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, College of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, P. R. China.
State Key Laboratory of Chemo/Biosensing and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
出版信息
Mikrochim Acta. 2024 Aug 3;191(8):503. doi: 10.1007/s00604-024-06559-y.
An upconversion fluorescence sensing platform was developed with upconversion nanoparticles (UCNPs) as energy donors and gold nanoparticles (AuNPs) as energy acceptors, based on the FRET principle. They were used for quantitative detection of uranyl ions (UO) by amplifying the signal of the hybrid chain reaction (HCR). When UO are introduced, the FRET between AuNPs and UCNPs can be modulated through a HCR in the presence of high concentrations of sodium chloride. This platform provides exceptional sensitivity, with a detection limit as low as 68 pM for UO recognition. We have successfully validated the reliability of this method by analyzing authentic water samples, achieving satisfactory recoveries (89.00%-112.50%) that are comparable to those of ICP-MS. These results indicate that the developed sensing platform has the capability to identify trace UO in complex environmental samples.
基于荧光共振能量转移（FRET）原理，开发了一种以上转换纳米粒子（UCNPs）作为能量供体、金纳米粒子（AuNPs）作为能量受体的上转换荧光传感平台。它们通过放大杂交链式反应（HCR）的信号用于定量检测铀酰离子（UO）。当引入UO时，在高浓度氯化钠存在下，AuNPs和UCNPs之间的FRET可通过HCR进行调节。该平台具有出色的灵敏度，UO识别的检测限低至68 pM。通过分析实际水样，我们成功验证了该方法的可靠性，获得了与电感耦合等离子体质谱法（ICP-MS）相当的令人满意的回收率（89.00%-112.50%）。这些结果表明，所开发的传感平台有能力识别复杂环境样品中的痕量UO。
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