Wang Dan, Zhang Li-Jie, Liu Ming-Hui, Du Fang-Fang, Shen Ze-Ya, He Longwei, Wang Li-Li
Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421001, China.
Department of Urology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, China.
J Hazard Mater. 2023 Jul 15;454:131497. doi: 10.1016/j.jhazmat.2023.131497. Epub 2023 Apr 25.
Uranium is one of the most important radionuclides but could also cause potential health risks to human beings due to its radioactive and chemical toxicity. It is an urgent task to develop a simple but efficient sensing platform for UO, the main existing form of uranium in environment. Herein, a rhodamine-functionalized carbon dots (o-CDs-Rho) was synthesized and applied for UO sensing through a simple but novel aggregation-enhanced FRET strategy. The weak FRET efficiency (16.2%) of o-CDs-Rho in dispersed solution is significantly enhanced (>77.2%) after UO triggered aggregation due to the increased number of rhodamine acceptors around each CDs from dispersed 80 to aggregated 2800. This is the first ratiometric fluorescence sensor with an inverse change of fluorescence intensity at dual emission wavelengths under single-wavelength excitation for UO. Under optimized experiment conditions, o-CDs-Rho nanosensor shows a low detection limit of 53 nM and excellent selectivity. Meanwhile, the as-prepared nanosensor also shows high reliability and stability. These excellent properties make it successful in detecting uranium content in real samples.
铀是最重要的放射性核素之一，但由于其放射性和化学毒性，也可能对人类造成潜在的健康风险。开发一种简单而高效的传感平台来检测环境中铀的主要存在形式UO，是一项紧迫的任务。在此，合成了一种罗丹明功能化的碳点（o-CDs-Rho），并通过一种简单而新颖的聚集增强荧光共振能量转移策略将其应用于UO传感。由于UO触发聚集后，每个碳点周围罗丹明受体的数量从分散状态下的80个增加到聚集状态下的2800个，o-CDs-Rho在分散溶液中的弱荧光共振能量转移效率（16.2%）显著提高（>77.2%）。这是首个在单波长激发下，在双发射波长处荧光强度呈反向变化的用于UO的比率荧光传感器。在优化的实验条件下，o-CDs-Rho纳米传感器显示出53 nM的低检测限和优异的选择性。同时，所制备的纳米传感器还具有高可靠性和稳定性。这些优异的性能使其成功用于检测实际样品中的铀含量。
