Graduate School of Jinzhou Medical University, Jinzhou, Liaoning Province, PR China; Central Laboratory, Linyi People's Hospital, Linyi, Shandong Province, PR China.
Department of Anesthesia, Linyi People's Hospital, Linyi, Shandong Province, PR China.
Seizure. 2024 Oct;121:70-77. doi: 10.1016/j.seizure.2024.07.014. Epub 2024 Jul 28.
Inflammation plays an important role in epilepsy. There is evidence for the relationship between proinflammatory cytokines and epilepsy. We aimed to detect the serum levels of multiple cytokines in epilepsy patients, looking for biological indicators, and providing a theoretical basis for the clinical diagnosis, treatment, and prognosis of epilepsy.
In this study, 30 patients with drug-resistant epilepsy (DRE), 30 patients with well-controlled epilepsy (WCE), and 29 healthy controls (HC) were enrolled. Multi-proinflammatory cytokines were measured by LUMINX multi-factor detection.
The levels of IL-1β, IL-7, IL-12, and IL-17 were significantly elevated, and the levels of CX3CL1 and ITAC were significantly decreased in epilepsy patients compared with healthy controls. Furthermore, the level of IL-17 was significantly higher in the DRE group compared to WCE. We also found the ratio of IL-7/CX3CL discriminates accurately between patients and controls, with a ROC Area Under the Curve (AUC) of 0.963 (P<0.001). The levels of IL-1β, IL-7, IL-12, and IL-17 in the DRE group were positively correlated with the National Hospital Seizure Severity Scale (NHS3) scores (IL-1β, P = 0.029; IL-12, P = 0.039; IL-17, P = 0.004). IL-17 was positively correlated with seizure frequency (P = 0.050), while ITAC was negatively correlated with seizure frequency (P = 0.012) and Sudden Unexpected Death in Epilepsy-3 (SUDEP-3) scores (P = 0.023).
IL-1β, IL-12, and IL-17 may be used to predict seizure severity and the IL-7/CX3CL1 ratio may be a candidate biomarker for predicting epileptic seizures. While CX3CL1 and ITAC play anti-epileptic effects, ITAC may be used to assess the risk of SUDEP.
炎症在癫痫中起重要作用。有证据表明促炎细胞因子与癫痫之间存在关系。我们旨在检测癫痫患者的多种细胞因子血清水平，寻找生物标志物，为癫痫的临床诊断、治疗和预后提供理论依据。
本研究纳入了 30 例耐药性癫痫（DRE）患者、30 例癫痫控制良好（WCE）患者和 29 名健康对照者（HC）。通过 LUMINX 多因子检测来测量多种促炎细胞因子。
与健康对照组相比，癫痫患者的 IL-1β、IL-7、IL-12 和 IL-17 水平显著升高，而 CX3CL1 和 ITAC 水平显著降低。此外，DRE 组的 IL-17 水平明显高于 WCE 组。我们还发现，IL-7/CX3CL1 的比值能准确地区分患者和对照组，ROC 曲线下面积（AUC）为 0.963（P<0.001）。DRE 组中 IL-1β、IL-7、IL-12 和 IL-17 的水平与国家癫痫严重程度量表（NHS3）评分呈正相关（IL-1β，P=0.029；IL-12，P=0.039；IL-17，P=0.004）。IL-17 与癫痫发作频率呈正相关（P=0.050），而 ITAC 与癫痫发作频率呈负相关（P=0.012）和癫痫猝死 3 （SUDEP-3）评分呈负相关（P=0.023）。
IL-1β、IL-12 和 IL-17 可能用于预测癫痫发作的严重程度，而 IL-7/CX3CL1 比值可能是预测癫痫发作的候选生物标志物。虽然 CX3CL1 和 ITAC 具有抗癫痫作用，但 ITAC 可能用于评估 SUDEP 的风险。
