Bodipy-Based highly sensitive hydrogen sulfide fluorescent probe and its fluorescence imaging in cells and zebrafish.

Hydrogen sulfide (HS) is a crucial endogenous gasotransmitter that plays a role in various physiological and pathological processes. Therefore, accurate and rapid monitoring of HS in organisms is highly significant for understanding the underlying pathological mechanisms and facilitating early diagnosis of related diseases. In this study, we developed a novel fluorescent probe, B-CHO-NO, based on a bodipy fluorophore, which exhibits excellent sensitivity and selectivity towards HS. The design of the probe exploits the nucleophilicity of HS by introducing a formyl group as the ortho-participating moiety, significantly enhancing the reaction rate with HS. In cellular and zebrafish models, the probe B-CHO-NO successfully achieved fluorescence imaging of endogenous and exogenous HS. The development of probe B-CHO-NO provides a powerful tool for biological studies of HS and diagnosis of related diseases.