Department of Public and Environmental Health, Polytechnic of Porto - School of Health (ESS), Porto, Portugal.
Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Porto, Portugal.
Adv Drug Deliv Rev. 2024 Sep;212:115420. doi: 10.1016/j.addr.2024.115420. Epub 2024 Aug 2.
The evaluation of the diffusion properties of different molecules in tissues is a subject of great interest in various fields, such as dermatology/cosmetology, clinical medicine, implantology and food preservation. In this review, a discussion of recent studies that used kinetic spectroscopy measurements to evaluate such diffusion properties in various tissues is made. By immersing ex vivo tissues in agents or by topical application of those agents in vivo, their diffusion properties can be evaluated by kinetic collimated transmittance or diffuse reflectance spectroscopy. Using this method, recent studies were able to discriminate the diffusion properties of agents between healthy and diseased tissues, especially in the cases of cancer and diabetes mellitus. In the case of cancer, it was also possible to evaluate an increase of 5% in the mobile water content from the healthy to the cancerous colorectal and kidney tissues. Considering the application of some agents to living organisms or food products to protect them from deterioration during low temperature preservation (cryopreservation), and knowing that such agent inclusion may be reversed, some studies in these fields are also discussed. Considering the broadband application of the optical spectroscopy evaluation of the diffusion properties of agents in tissues and the physiological diagnostic data that such method can acquire, further studies concerning the optimization of fruit sweetness or evaluation of poison diffusion in tissues or antidote application for treatment optimization purposes are indicated as future perspectives.
不同分子在组织中的扩散性质的评估是皮肤学/美容学、临床医学、植入物学和食品保鲜等各个领域非常关注的课题。在这篇综述中，讨论了最近使用动力学光谱测量来评估各种组织中这种扩散性质的研究。通过将离体组织浸入试剂中，或通过体内局部应用这些试剂，可以通过动力学准直透射或漫反射光谱来评估其扩散性质。使用这种方法，最近的研究能够区分健康组织和患病组织中试剂的扩散性质，特别是在癌症和糖尿病的情况下。在癌症的情况下，还可以从健康组织到癌症直肠和肾脏组织评估移动水含量增加 5%。考虑到一些试剂应用于生物体或食品产品中，以防止它们在低温保存（冷冻保存）过程中变质，并且知道这种试剂的包含可能会被逆转，因此也讨论了这些领域的一些研究。考虑到光学光谱对组织中试剂扩散性质的广泛应用以及这种方法可以获得的生理诊断数据，进一步的研究表明，优化水果甜度或评估组织中毒物扩散以及应用解毒剂来优化治疗效果是未来的研究方向。
